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• W1988836828 abstract "Effects of pH and dilution on sperm motility were examined using the African clawed frog, Xenopus laevis (Daudin, 1802), as a model species. Sperm removed from adult X. laevis males were stored on ice in DeBoer's solution, rendering them immotile until activation by dilution. A series of pHs ranging from 5.5 to 7.8 and a dilution series ranging from 1:1 to 5:1 (diluent : sperm solution) were examined. Motility was assessed by constructing sperm track maps for individual spermatozoa using video recordings. pH did not significantly affect the percentage of spermatozoa with motility; however, pH 7.0 produced observably higher motility than other pH treatments. Velocities and velocity ratios were not significantly affected by the various pH treatments. A solution with a 3:1 dilution ratio resulted in the highest percentage of sperm with motility (55.3 ± 8.5%) and the highest curvilinear velocity (approximately 65 µm/s). Average path velocity, as well as the linearity and wobble of the sperm track, were also significantly affected by dilution. The results indicate that the chemistry of the freshwater environment in which X. laevis sperm must swim to the eggs may be important for successful fertilization to take place." @default.
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• W1988836828 date "2004-04-01" @default.
• W1988836828 modified "2023-10-04" @default.
• W1988836828 title "Effects of pH and dilution on African clawed frog (<i>Xenopus laevis</i>) sperm motility" @default.
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• W1988836828 doi "https://doi.org/10.1139/z04-021" @default.
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