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- W1988902221 abstract "The kinetics of secretion of proteins by Leishmania braziliensis was followed by incorporation of [3H]leucine into macromolecules produced by the cells which are released into the growth medium. About 10% of the total protein synthesized by actively growing cells is secreted. Cycloheximide (100 μg/ml) and puromycin (0.5 mM) inhibited the incorporation of labelled leucine by 85 and 99%, respectively. The secreted proteins do not seem to results from cell lysis since, first, the kinetics of production are linear and, secondly, less than 1% the thymidine or uridine incorporated by the cells is found in the medium. Cells grownh with [3H]leucine and then transferred to fresh medium show two phases of secretion. During the first six hours, it is slow and reaches a plateau. The release increases about ten-fold during the next six hours. An analysis of the secreted material showed that following precipitation with methanol and sodium acetate, three isotopically labelled peaks were eluted from Sephadex G-120–150. The first of these, containing 50% of the radioactivity, did not react with anti-leishmanial serum, while the last two did. Since the last two fractions could be labelled with [3H]glucosamine as well as [3H]leucine it is suggested that they are glycoprotein in nature and are similar to the products released by other species of Leishmania." @default.
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- W1988902221 date "1980-01-01" @default.
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- W1988902221 title "Production and secretion of Leishmania braziliensis proteins" @default.
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- W1988902221 doi "https://doi.org/10.1016/0166-6851(80)90013-4" @default.
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