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- W1988908300 abstract "Molecular diagnostics in tuberculosis has enabled rapid detection of Mycobacterium tuberculosis complex in clinical specimens, identification of mycobacterial species, detection of drug resistance, and typing for epidemiological investigation. In the laboratory diagnosis of tuberculosis, the nucleic acid amplification (NAA) test is rapid and specific but not as sensitive as culture of mycobacteria. The primary determinant of successful NAA testing for tuberculosis depends on the shedding of mycobacterial DNA in secretions from caseating granulomas and its dissemination into sterile body fluids or tissue biopsies. In multibacillary diseases with a high mycobacterial load, a positive Ziehl–Neelsen smear with a positive NAA test is diagnostic of active tuberculosis, whereas a positive Ziehl–Neelsen smear with a negative NAA test in the absence of inhibitors would indicate nontuberculous mycobacterial disease. The role of the NAA test is more important in paucibacillary diseases with low mycobacterial loads. The presence of polymerase chain reaction (PCR) inhibitors, however, especially in extrapulmonary specimens, may produce false-negative results. Although this problem can be overcome to some extent by extra extraction steps, the additional processing invariably leads to the loss of mycobacterial DNA. To circumvent this problem, a brief culture augmentation step is carried out before the NAA test is performed, which can enhance the mycobacterial load while concomitantly diluting inhibitors, thereby maintaining the sensitivity of the test without excessively increasing turnaround time." @default.
- W1988908300 created "2016-06-24" @default.
- W1988908300 creator A5032806661 @default.
- W1988908300 creator A5052262194 @default.
- W1988908300 creator A5074675036 @default.
- W1988908300 date "2005-11-01" @default.
- W1988908300 modified "2023-10-01" @default.
- W1988908300 title "Molecular diagnostics in tuberculosis" @default.
- W1988908300 cites W11072214 @default.
- W1988908300 cites W1455086614 @default.
- W1988908300 cites W1520301297 @default.
- W1988908300 cites W1591522501 @default.
- W1988908300 cites W1640293576 @default.
- W1988908300 cites W1760315734 @default.
- W1988908300 cites W1793129498 @default.
- W1988908300 cites W1831135453 @default.
- W1988908300 cites W1850823861 @default.
- W1988908300 cites W1858667347 @default.
- W1988908300 cites W1884882993 @default.
- W1988908300 cites W1906071890 @default.
- W1988908300 cites W1970910747 @default.
- W1988908300 cites W1973650123 @default.
- W1988908300 cites W1973853438 @default.
- W1988908300 cites W1980051361 @default.
- W1988908300 cites W1982473418 @default.
- W1988908300 cites W1985972796 @default.
- W1988908300 cites W1987291561 @default.
- W1988908300 cites W1992711453 @default.
- W1988908300 cites W2000310890 @default.
- W1988908300 cites W2002132219 @default.
- W1988908300 cites W2005769920 @default.
- W1988908300 cites W2006103746 @default.
- W1988908300 cites W2007349523 @default.
- W1988908300 cites W2010083350 @default.
- W1988908300 cites W2017629658 @default.
- W1988908300 cites W2031491635 @default.
- W1988908300 cites W2032853169 @default.
- W1988908300 cites W2033164773 @default.
- W1988908300 cites W2033296288 @default.
- W1988908300 cites W2042616838 @default.
- W1988908300 cites W2044353203 @default.
- W1988908300 cites W2045008561 @default.
- W1988908300 cites W2045398283 @default.
- W1988908300 cites W2048220849 @default.
- W1988908300 cites W2058764691 @default.
- W1988908300 cites W2065684227 @default.
- W1988908300 cites W2070272773 @default.
- W1988908300 cites W2073320344 @default.
- W1988908300 cites W2075196372 @default.
- W1988908300 cites W2078882321 @default.
- W1988908300 cites W2085765610 @default.
- W1988908300 cites W2088922035 @default.
- W1988908300 cites W2093116614 @default.
- W1988908300 cites W2094590640 @default.
- W1988908300 cites W2096862686 @default.
- W1988908300 cites W2097086169 @default.
- W1988908300 cites W2100590776 @default.
- W1988908300 cites W2103085505 @default.
- W1988908300 cites W2104252646 @default.
- W1988908300 cites W2104417772 @default.
- W1988908300 cites W2104550086 @default.
- W1988908300 cites W2105970723 @default.
- W1988908300 cites W2106477727 @default.
- W1988908300 cites W2108609455 @default.
- W1988908300 cites W2109232120 @default.
- W1988908300 cites W2114090343 @default.
- W1988908300 cites W2114888478 @default.
- W1988908300 cites W2117484165 @default.
- W1988908300 cites W2119453720 @default.
- W1988908300 cites W2119745045 @default.
- W1988908300 cites W2120659509 @default.
- W1988908300 cites W2124376346 @default.
- W1988908300 cites W2124663438 @default.
- W1988908300 cites W212636303 @default.
- W1988908300 cites W2126619965 @default.
- W1988908300 cites W2128067448 @default.
- W1988908300 cites W2129754454 @default.
- W1988908300 cites W2131732398 @default.
- W1988908300 cites W2131897033 @default.
- W1988908300 cites W2134981183 @default.
- W1988908300 cites W2139032425 @default.
- W1988908300 cites W2141627564 @default.
- W1988908300 cites W2141641782 @default.
- W1988908300 cites W2142006729 @default.
- W1988908300 cites W2143076632 @default.
- W1988908300 cites W2146067328 @default.
- W1988908300 cites W2147747903 @default.
- W1988908300 cites W2150106241 @default.
- W1988908300 cites W2151164386 @default.
- W1988908300 cites W2152478949 @default.
- W1988908300 cites W2152906249 @default.
- W1988908300 cites W2153285588 @default.
- W1988908300 cites W2155167411 @default.
- W1988908300 cites W2157021376 @default.
- W1988908300 cites W2158023121 @default.
- W1988908300 cites W2160543289 @default.
- W1988908300 cites W2162592621 @default.
- W1988908300 cites W2162889850 @default.