Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988929994> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W1988929994 endingPage "A696" @default.
- W1988929994 startingPage "A696" @default.
- W1988929994 abstract "BACKGROUND: in a hepa ic ischer fi>mperfusion (I/R) i~jury, massive Joss of thrombomo~ dulin (TM) t o m the sinusoida/endothelia] ceils aright be playing a central role in developing a liver damage especially ~l a warm ischemia of the graft trom non-Jmambeating donor Genet c modibcatior: o the liver graft to express exogenous TM might thus conmbure to an improvement of hepatic I/R ir~jury METttODS: Three groups ot 9 rats each underwent intra-venous in~ec ion of marke~ LacZ adeuovirus vector in Groupl (AxCAlacZ lX 109 cfuJ m[), human IM adenovims vector m Group 2 (AxCAhTM, I X 10 cfu/nt|), or normal saline in Grocp 3 (1 ml) For y-eigbt hrs atier gone transler, rats were anesthetized by ether and subjected to hepat c wa~-n ischerma of 30 rain. In all the groups, each 3 rats were sacrihced at 12 hrs, 24 his, and 7 days alter reperhision, to obtain the serum and hepatic tissue samples Serum samples were used to determine hepatic enzyme kvels, hyal'aroinc acid levels anti o t h e s Serum and hepatic tissue levels oi h-TM were also determined by an EL1SA (enzyme linked iramuno-.sorbent assay), Hepatic expression of Marker LacZ and hTM were also evaluated by a X-gal staining and immunnhistochemical staimng. RESULTS: Expression of marker LacZ (X-gai staining) in Group 1 and h-TM (immunohistochemical staining and R]'-PCR) m Group 2 were detected in the hver, until 7 days after hepatic ischemia Hepatic tissce blood flow (Laser Doppler) showed no difl}ren~e between the groups immediately niter reperfusion Serum ALT levels (205-+ 55 ILIA), hyaluronic acid (0 1 ] 8 2 007 ~gnll), and hepatic tissue blood flow were sigmficantly improved onIy in the hTM transiected Group 2, at 12 hrs alter repe~usion, CONCLUSION: These findings thus s~.ggested that adeuovi:rus mediated gone transfer oi h-TM helped to attenuate a liver damage, n the r a model ot warn ischemic liver injury," @default.
- W1988929994 created "2016-06-24" @default.
- W1988929994 creator A5008693834 @default.
- W1988929994 creator A5010147556 @default.
- W1988929994 creator A5017342176 @default.
- W1988929994 creator A5051306531 @default.
- W1988929994 date "2003-04-01" @default.
- W1988929994 modified "2023-10-18" @default.
- W1988929994 title "Adenovirus mediated gene transfer of human thrombomodulin in the rat model of warm ischemic liver injury" @default.
- W1988929994 doi "https://doi.org/10.1016/s0016-5085(03)83519-9" @default.
- W1988929994 hasPublicationYear "2003" @default.
- W1988929994 type Work @default.
- W1988929994 sameAs 1988929994 @default.
- W1988929994 citedByCount "0" @default.
- W1988929994 crossrefType "journal-article" @default.
- W1988929994 hasAuthorship W1988929994A5008693834 @default.
- W1988929994 hasAuthorship W1988929994A5010147556 @default.
- W1988929994 hasAuthorship W1988929994A5017342176 @default.
- W1988929994 hasAuthorship W1988929994A5051306531 @default.
- W1988929994 hasBestOaLocation W19889299941 @default.
- W1988929994 hasConcept C104317684 @default.
- W1988929994 hasConcept C126322002 @default.
- W1988929994 hasConcept C185592680 @default.
- W1988929994 hasConcept C201267052 @default.
- W1988929994 hasConcept C2776637226 @default.
- W1988929994 hasConcept C2777292125 @default.
- W1988929994 hasConcept C2993714741 @default.
- W1988929994 hasConcept C3018928802 @default.
- W1988929994 hasConcept C541997718 @default.
- W1988929994 hasConcept C55493867 @default.
- W1988929994 hasConcept C71924100 @default.
- W1988929994 hasConcept C89560881 @default.
- W1988929994 hasConceptScore W1988929994C104317684 @default.
- W1988929994 hasConceptScore W1988929994C126322002 @default.
- W1988929994 hasConceptScore W1988929994C185592680 @default.
- W1988929994 hasConceptScore W1988929994C201267052 @default.
- W1988929994 hasConceptScore W1988929994C2776637226 @default.
- W1988929994 hasConceptScore W1988929994C2777292125 @default.
- W1988929994 hasConceptScore W1988929994C2993714741 @default.
- W1988929994 hasConceptScore W1988929994C3018928802 @default.
- W1988929994 hasConceptScore W1988929994C541997718 @default.
- W1988929994 hasConceptScore W1988929994C55493867 @default.
- W1988929994 hasConceptScore W1988929994C71924100 @default.
- W1988929994 hasConceptScore W1988929994C89560881 @default.
- W1988929994 hasIssue "4" @default.
- W1988929994 hasLocation W19889299941 @default.
- W1988929994 hasOpenAccess W1988929994 @default.
- W1988929994 hasPrimaryLocation W19889299941 @default.
- W1988929994 hasRelatedWork W1879733234 @default.
- W1988929994 hasRelatedWork W1988929994 @default.
- W1988929994 hasRelatedWork W2050080970 @default.
- W1988929994 hasRelatedWork W2135239906 @default.
- W1988929994 hasRelatedWork W2354901326 @default.
- W1988929994 hasRelatedWork W2356139802 @default.
- W1988929994 hasRelatedWork W2374854293 @default.
- W1988929994 hasRelatedWork W2376837706 @default.
- W1988929994 hasRelatedWork W2384095809 @default.
- W1988929994 hasRelatedWork W2768963646 @default.
- W1988929994 hasVolume "124" @default.
- W1988929994 isParatext "false" @default.
- W1988929994 isRetracted "false" @default.
- W1988929994 magId "1988929994" @default.
- W1988929994 workType "article" @default.