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- W1988948397 endingPage "410" @default.
- W1988948397 startingPage "394" @default.
- W1988948397 abstract "Abstract The intercellular movement of plant viruses requires both viral and host proteins. Previous studies have demonstrated that the frame-shift protein P3N-PIPO (for the protein encoded by the open reading frame [ORF] containing 5′-terminus of P3 and a +2 frame-shift ORF called Pretty Interesting Potyviridae ORF and embedded in the P3) and CYLINDRICAL INCLUSION (CI) proteins were required for potyvirus cell-to-cell movement. Here, we provide genetic evidence showing that a Tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) mutant carrying a truncated PIPO domain of 58 amino acid residues could move between cells and induce systemic infection in Nicotiana benthamiana plants; mutants carrying a PIPO domain of seven, 20, or 43 amino acid residues failed to move between cells and cause systemic infection in this host plant. Interestingly, the movement-defective mutants produced progeny that eliminated the previously introduced stop codons and thus restored their systemic movement ability. We also present evidence showing that a developmentally regulated plasma membrane protein of N. benthamiana (referred to as NbDREPP) interacted with both P3N-PIPO and CI of the movement-competent TVBMV. The knockdown of NbDREPP gene expression in N. benthamiana impeded the cell-to-cell movement of TVBMV. NbDREPP was shown to colocalize with TVBMV P3N-PIPO and CI at plasmodesmata (PD) and traffic to PD via the early secretory pathway and the actomyosin motility system. We also show that myosin XI-2 is specially required for transporting NbDREPP to PD. In conclusion, NbDREPP is a key host protein within the early secretory pathway and the actomyosin motility system that interacts with two movement proteins and influences virus movement." @default.
- W1988948397 created "2016-06-24" @default.
- W1988948397 creator A5042690169 @default.
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- W1988948397 creator A5072692179 @default.
- W1988948397 creator A5081805002 @default.
- W1988948397 creator A5090975713 @default.
- W1988948397 date "2014-12-24" @default.
- W1988948397 modified "2023-10-14" @default.
- W1988948397 title "DEVELOPMENTALLY REGULATED PLASMA MEMBRANE PROTEIN of <i>Nicotiana benthamiana</i> Contributes to Potyvirus Movement and Transports to Plasmodesmata via the Early Secretory Pathway and the Actomyosin System " @default.
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- W1988948397 doi "https://doi.org/10.1104/pp.114.252734" @default.
- W1988948397 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4326756" @default.
- W1988948397 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25540331" @default.
- W1988948397 hasPublicationYear "2014" @default.
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