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- W1988996843 abstract "We investigate the working principle of a novel type of microfluidic sandwich immunoassay, as used for the detection of biomarkers. The heterogeneous assay is based on the specific interactions between an array of functionalized superparamagnetic beads and a flow of secondary superparamagnetic beads that carry the antigens and are simultaneously used as detection labels. We identify the main forces governing the immunoassay performance and develop a combined finite element method/analytical model to predict and control these forces. The clue for the improved assay specificity is in the fine-tuning of inter-bead magnetic dipolar and microfluidic viscous forces, which allows strongly reducing non-specific interactions, while enhancing the specific formation of immunocomplexes. We exploit our theoretical model to explain the enhanced sensitivity of magnetic bead-based immunoassay experiments performed in microfluidic chips." @default.
- W1988996843 created "2016-06-24" @default.
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- W1988996843 date "2013-08-09" @default.
- W1988996843 modified "2023-10-03" @default.
- W1988996843 title "Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation" @default.
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- W1988996843 doi "https://doi.org/10.1063/1.4817663" @default.
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