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• W1989048788 abstract "Earlier antimonate techniques for electron microscopic localization of sodium were applied to isolated islets of Langerhans obtained by partial collagenase digestion of mouse and rabbit pancreas. Incubation of islets in low or high glucose media followed by fixation in either (a) glutaraldehyde-osmium-antimonate mixtures or (b) osmium-antimonate mixtures resulted in deposition of precipitation particles in association with organelles directly involved in insulin secretion: beta granules (saccules) and cell membranes. Precipitation particles increased at these two cell sites following glucose incubation. X-ray microprobe analysis of precipitation particles using the AEI-EMMA-4 analytical microscope revealed the presence of other ions in addition to sodium, confirming the nonspecific nature of the antimonate technique. The predominant cation associated with the beta granule saccule and cell membrane was calcium, and the increased precipitation following glucose stimulation suggests an active role of this cation in the insulin secretion process as proposed earlier by biochemical methods." @default.
• W1989048788 created "2016-06-24" @default.
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• W1989048788 date "1973-01-01" @default.
• W1989048788 modified "2023-10-13" @default.
• W1989048788 title "The electron microscopic localization of cations to pancreatic islets of langerhans and their possible role in insulin secretion" @default.
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• W1989048788 doi "https://doi.org/10.1016/s0022-5320(73)90058-0" @default.
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