Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989088823> ?p ?o ?g. }
- W1989088823 endingPage "2456" @default.
- W1989088823 startingPage "2452" @default.
- W1989088823 abstract "To study the potential of liposome vaccines in the clinical prevention of ALV-J, the effect of recombinant gp85 protein of subgroup J avian leukosis virus (ALV-J) entrapped by liposomes in chickens against ALV-J infection was investigated in this paper. A recombinant plasmid (PET28a-gp85) containing the PET28a vector and gp85 gene was constructed and then expressed in Rosetta (DE3) cells with 0.5 mM IPTG to produce recombinant gp85 proteins that could be entrapped by liposomes through reverse-phase evaporation. The chickens were inoculated intramuscularly either once or twice with the liposomes or with Freund's adjuvant emulsion containing recombinant gp85 protein. Sixty chickens were raised to one week old for the first inoculation and to three weeks old for the second inoculation. Chickens raised to five weeks old were challenged with a 102.4 50% tissue culture infective dose (TCID50) of ALV-J. Blood samples were collected from each chicken at weekly intervals for serum antibody and viremia analyses. Changes in serum antibodies showed that positive serum antibodies (S/P value >0.6) could be induced in all groups regardless of the frequency of inoculation but improved significantly in the twice-inoculated groups. As well, high levels of antibodies emerged earlier in the Freund's adjuvant groups but persisted longer in the liposome groups. Detection of viremia indicated that the liposomes provide better protection against ALV-J than Freund's adjuvant emulsion and that this protection is directly influenced by serum antibody levels. Overall, this study reveals the potential of liposome vaccines containing recombinant gp85 protein in the clinical prevention of ALV-J." @default.
- W1989088823 created "2016-06-24" @default.
- W1989088823 creator A5002849906 @default.
- W1989088823 creator A5005828971 @default.
- W1989088823 creator A5008229118 @default.
- W1989088823 creator A5019949353 @default.
- W1989088823 creator A5022590540 @default.
- W1989088823 creator A5031096137 @default.
- W1989088823 creator A5060413120 @default.
- W1989088823 creator A5066122148 @default.
- W1989088823 creator A5089753752 @default.
- W1989088823 creator A5089865552 @default.
- W1989088823 date "2014-05-01" @default.
- W1989088823 modified "2023-10-16" @default.
- W1989088823 title "Liposomes containing recombinant gp85 protein vaccine against ALV-J in chickens" @default.
- W1989088823 cites W1483282953 @default.
- W1989088823 cites W1777091040 @default.
- W1989088823 cites W1946903217 @default.
- W1989088823 cites W1963895695 @default.
- W1989088823 cites W1969175740 @default.
- W1989088823 cites W1982661859 @default.
- W1989088823 cites W1991196777 @default.
- W1989088823 cites W1994064910 @default.
- W1989088823 cites W2006021991 @default.
- W1989088823 cites W2011302465 @default.
- W1989088823 cites W2019502257 @default.
- W1989088823 cites W2023469796 @default.
- W1989088823 cites W2027611504 @default.
- W1989088823 cites W2059780572 @default.
- W1989088823 cites W2061510929 @default.
- W1989088823 cites W2062730112 @default.
- W1989088823 cites W2064062918 @default.
- W1989088823 cites W2067141568 @default.
- W1989088823 cites W2068359650 @default.
- W1989088823 cites W2074068865 @default.
- W1989088823 cites W2075559837 @default.
- W1989088823 cites W2079877086 @default.
- W1989088823 cites W2133464417 @default.
- W1989088823 cites W2135221251 @default.
- W1989088823 cites W2165837635 @default.
- W1989088823 cites W2177342405 @default.
- W1989088823 cites W2293286604 @default.
- W1989088823 cites W2315724696 @default.
- W1989088823 cites W3025036998 @default.
- W1989088823 doi "https://doi.org/10.1016/j.vaccine.2014.02.091" @default.
- W1989088823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24625339" @default.
- W1989088823 hasPublicationYear "2014" @default.
- W1989088823 type Work @default.
- W1989088823 sameAs 1989088823 @default.
- W1989088823 citedByCount "20" @default.
- W1989088823 countsByYear W19890888232015 @default.
- W1989088823 countsByYear W19890888232016 @default.
- W1989088823 countsByYear W19890888232017 @default.
- W1989088823 countsByYear W19890888232018 @default.
- W1989088823 countsByYear W19890888232019 @default.
- W1989088823 countsByYear W19890888232020 @default.
- W1989088823 countsByYear W19890888232022 @default.
- W1989088823 countsByYear W19890888232023 @default.
- W1989088823 crossrefType "journal-article" @default.
- W1989088823 hasAuthorship W1989088823A5002849906 @default.
- W1989088823 hasAuthorship W1989088823A5005828971 @default.
- W1989088823 hasAuthorship W1989088823A5008229118 @default.
- W1989088823 hasAuthorship W1989088823A5019949353 @default.
- W1989088823 hasAuthorship W1989088823A5022590540 @default.
- W1989088823 hasAuthorship W1989088823A5031096137 @default.
- W1989088823 hasAuthorship W1989088823A5060413120 @default.
- W1989088823 hasAuthorship W1989088823A5066122148 @default.
- W1989088823 hasAuthorship W1989088823A5089753752 @default.
- W1989088823 hasAuthorship W1989088823A5089865552 @default.
- W1989088823 hasConcept C104317684 @default.
- W1989088823 hasConcept C159047783 @default.
- W1989088823 hasConcept C159654299 @default.
- W1989088823 hasConcept C180032290 @default.
- W1989088823 hasConcept C185154212 @default.
- W1989088823 hasConcept C203014093 @default.
- W1989088823 hasConcept C2522874641 @default.
- W1989088823 hasConcept C2776185481 @default.
- W1989088823 hasConcept C2777863537 @default.
- W1989088823 hasConcept C40767141 @default.
- W1989088823 hasConcept C55493867 @default.
- W1989088823 hasConcept C86803240 @default.
- W1989088823 hasConcept C89423630 @default.
- W1989088823 hasConceptScore W1989088823C104317684 @default.
- W1989088823 hasConceptScore W1989088823C159047783 @default.
- W1989088823 hasConceptScore W1989088823C159654299 @default.
- W1989088823 hasConceptScore W1989088823C180032290 @default.
- W1989088823 hasConceptScore W1989088823C185154212 @default.
- W1989088823 hasConceptScore W1989088823C203014093 @default.
- W1989088823 hasConceptScore W1989088823C2522874641 @default.
- W1989088823 hasConceptScore W1989088823C2776185481 @default.
- W1989088823 hasConceptScore W1989088823C2777863537 @default.
- W1989088823 hasConceptScore W1989088823C40767141 @default.
- W1989088823 hasConceptScore W1989088823C55493867 @default.
- W1989088823 hasConceptScore W1989088823C86803240 @default.
- W1989088823 hasConceptScore W1989088823C89423630 @default.
- W1989088823 hasIssue "21" @default.
- W1989088823 hasLocation W19890888231 @default.
- W1989088823 hasLocation W19890888232 @default.