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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989181330> ?p ?o ?g. }

• W1989181330 endingPage "2019" @default.
• W1989181330 startingPage "2014" @default.
• W1989181330 abstract "Gene therapy shows promise for treating prostate cancer and has been evaluated in several clinical trials. A major challenge that remains is to establish a method for verifying transgene activity in situ. The lacZ gene encoding beta-galactosidase historically has been the most popular reporter gene for molecular biology. We have designed a 19F NMR approach to reveal lacZ gene expression by assessing beta-galactosidase (beta-gal) activity in vivo. The substrate 2-fluoro-4-nitrophenyl beta-D-galactopyranoside (OFPNPG) is readily hydrolyzed by beta-gal with a corresponding decrease in the 19F-NMR signal from OFPNPG and the appearance of a new signal shifted 4-6 ppm upfield from the aglycone 2-fluoro-4-nitrophenol (OFPNP). We report proof of principle in cultures of PC3 prostate cancer cells using 19F NMR spectroscopy and 19F chemical shift imaging. More importantly, we demonstrate for the first time the ability to differentiate wild-type and lacZ-expressing prostate tumor xenografts in mice using this approach." @default.
• W1989181330 created "2016-06-24" @default.
• W1989181330 creator A5041399302 @default.
• W1989181330 creator A5063481044 @default.
• W1989181330 creator A5081824220 @default.
• W1989181330 creator A5090381580 @default.
• W1989181330 date "2007-03-09" @default.
• W1989181330 modified "2023-10-16" @default.
• W1989181330 title "¹⁹ F‐NMR detection of <i>lacZ</i> gene expression <i>via</i> the enzymic hydrolysis of 2‐fluoro‐4‐nitrophenyl β‐D‐galactopyranoside <i>in vivo</i> in PC3 prostate tumor xenografts in the mouse ¹" @default.
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• W1989181330 doi "https://doi.org/10.1096/fj.06-7366lsf" @default.
• W1989181330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17351127" @default.
• W1989181330 hasPublicationYear "2007" @default.
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