FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989224728> ?p ?o ?g. }

• W1989224728 endingPage "5732" @default.
• W1989224728 startingPage "5727" @default.
• W1989224728 abstract "Here we report the structural characterization of the product formed from the reaction between hydroethidine (HE) and superoxide (O(2)(.-)). By using mass spectral and NMR techniques, the chemical structure of this product was determined as 2-hydroxyethidium (2-OH-E(+)). By using an authentic standard, we developed an HPLC approach to detect and quantitate the reaction product of HE and O(2)(.-) formed in bovine aortic endothelial cells after treatment with menadione or antimycin A to induce intracellular reactive oxygen species. Concomitantly, we used a spin trap, 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BMPO), to detect and identify the structure of reactive oxygen species formed. BMPO trapped the O(2)(.-) that formed extracellularly and was detected as the BMPO-OH adduct during use of the EPR technique. BMPO, being cell-permeable, inhibited the intracellular formation of 2-OH-E(+). However, the intracellular BMPO spin adduct was not detected. The definitive characterization of the reaction product of O(2)(.-) with HE described here forms the basis of an unambiguous assay for intracellular detection and quantitation of O(2)(.-). Analysis of the fluorescence characteristics of ethidium (E(+)) and 2-OH-E(+) strongly suggests that the currently available fluorescence methodology is not suitable for quantitating intracellular O(2)(.-). We conclude that the HPLC/fluorescence assay using HE as a probe is more suitable [corrected] for detecting intracellular O(2)(.-)." @default.
• W1989224728 created "2016-06-24" @default.
• W1989224728 creator A5001723877 @default.
• W1989224728 creator A5009303429 @default.
• W1989224728 creator A5035372773 @default.
• W1989224728 creator A5035666886 @default.
• W1989224728 creator A5076520162 @default.
• W1989224728 creator A5088738836 @default.
• W1989224728 creator A5089152409 @default.
• W1989224728 date "2005-04-11" @default.
• W1989224728 modified "2023-10-05" @default.
• W1989224728 title "Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence" @default.
• W1989224728 cites W1498711742 @default.
• W1989224728 cites W1925910300 @default.
• W1989224728 cites W1969260760 @default.
• W1989224728 cites W1970793703 @default.
• W1989224728 cites W1981783025 @default.
• W1989224728 cites W1997474765 @default.
• W1989224728 cites W2005452996 @default.
• W1989224728 cites W2009583207 @default.
• W1989224728 cites W2020600203 @default.
• W1989224728 cites W2057683250 @default.
• W1989224728 cites W2058269483 @default.
• W1989224728 cites W2072910428 @default.
• W1989224728 cites W2075579265 @default.
• W1989224728 cites W2075708068 @default.
• W1989224728 cites W2083714336 @default.
• W1989224728 cites W2087863751 @default.
• W1989224728 cites W2102202839 @default.
• W1989224728 cites W2107045256 @default.
• W1989224728 cites W2108843757 @default.
• W1989224728 cites W2133739669 @default.
• W1989224728 cites W2135633069 @default.
• W1989224728 cites W2158918409 @default.
• W1989224728 cites W2167866149 @default.
• W1989224728 cites W2171192249 @default.
• W1989224728 cites W2188526507 @default.
• W1989224728 cites W2188751783 @default.
• W1989224728 doi "https://doi.org/10.1073/pnas.0501719102" @default.
• W1989224728 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/556312" @default.
• W1989224728 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15824309" @default.
• W1989224728 hasPublicationYear "2005" @default.
• W1989224728 type Work @default.
• W1989224728 sameAs 1989224728 @default.
• W1989224728 citedByCount "511" @default.
• W1989224728 countsByYear W19892247282012 @default.
• W1989224728 countsByYear W19892247282013 @default.
• W1989224728 countsByYear W19892247282014 @default.
• W1989224728 countsByYear W19892247282015 @default.
• W1989224728 countsByYear W19892247282016 @default.
• W1989224728 countsByYear W19892247282017 @default.
• W1989224728 countsByYear W19892247282018 @default.
• W1989224728 countsByYear W19892247282019 @default.
• W1989224728 countsByYear W19892247282020 @default.
• W1989224728 countsByYear W19892247282021 @default.
• W1989224728 countsByYear W19892247282022 @default.
• W1989224728 countsByYear W19892247282023 @default.
• W1989224728 crossrefType "journal-article" @default.
• W1989224728 hasAuthorship W1989224728A5001723877 @default.
• W1989224728 hasAuthorship W1989224728A5009303429 @default.
• W1989224728 hasAuthorship W1989224728A5035372773 @default.
• W1989224728 hasAuthorship W1989224728A5035666886 @default.
• W1989224728 hasAuthorship W1989224728A5076520162 @default.
• W1989224728 hasAuthorship W1989224728A5088738836 @default.
• W1989224728 hasAuthorship W1989224728A5089152409 @default.
• W1989224728 hasBestOaLocation W19892247281 @default.
• W1989224728 hasConcept C108204754 @default.
• W1989224728 hasConcept C121332964 @default.
• W1989224728 hasConcept C12554922 @default.
• W1989224728 hasConcept C178790620 @default.
• W1989224728 hasConcept C179998833 @default.
• W1989224728 hasConcept C181199279 @default.
• W1989224728 hasConcept C185592680 @default.
• W1989224728 hasConcept C187961010 @default.
• W1989224728 hasConcept C2780795997 @default.
• W1989224728 hasConcept C43617362 @default.
• W1989224728 hasConcept C46141821 @default.
• W1989224728 hasConcept C48349386 @default.
• W1989224728 hasConcept C55493867 @default.
• W1989224728 hasConcept C62520636 @default.
• W1989224728 hasConcept C79879829 @default.
• W1989224728 hasConcept C86803240 @default.
• W1989224728 hasConcept C91881484 @default.
• W1989224728 hasConceptScore W1989224728C108204754 @default.
• W1989224728 hasConceptScore W1989224728C121332964 @default.
• W1989224728 hasConceptScore W1989224728C12554922 @default.
• W1989224728 hasConceptScore W1989224728C178790620 @default.
• W1989224728 hasConceptScore W1989224728C179998833 @default.
• W1989224728 hasConceptScore W1989224728C181199279 @default.
• W1989224728 hasConceptScore W1989224728C185592680 @default.
• W1989224728 hasConceptScore W1989224728C187961010 @default.
• W1989224728 hasConceptScore W1989224728C2780795997 @default.
• W1989224728 hasConceptScore W1989224728C43617362 @default.
• W1989224728 hasConceptScore W1989224728C46141821 @default.
• W1989224728 hasConceptScore W1989224728C48349386 @default.
• W1989224728 hasConceptScore W1989224728C55493867 @default.
• W1989224728 hasConceptScore W1989224728C62520636 @default.
• W1989224728 hasConceptScore W1989224728C79879829 @default.

• next