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- W1989285427 abstract "The position of the phosphate group on the ribose of nucleoside monophosphates markedly influences their binding behavior with basic polyamino acids, such as poly-l-lysine and poly-l-arginine. Equilibrium dialysis of adenine and guanine derivatives has revealed that the binding of 5′- and 3′-ribonucleotides (group I, possessing a free 2′OH group) to basic polyamino acids is clearly different from that of 2′-ribo and 5′-deoxyribonucleotides (group II, the 2′OH group is blocked and absent, respect.). At low nucleotide to poly-l-lysine ratios, where the binding corresponds to the Langmuir isotherm, the binding constants of group I are smaller than those of group II. At higher nucleotide to polypeptide ratios the binding is cooperative and accompanied by a phase transition: guanine derivatives of group I show precipitation with poly-l-lysine at 0′, whereas with group II a gel-like phase is separated; with poly-l-arginine only precipitation occurs. Analysis of the cooperative binding with guanine nucleotides and poly-l-arginine revealed that group I possesses considerably higher nearest neighbour interaction energies than group II. The stacking coefficients decrease in the order 3′-GMP > 5′-GMP > 5′-dGMP > 2′-GMP. The implications of these results are discussed." @default.
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- W1989285427 date "1970-04-01" @default.
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- W1989285427 title "The Influence of the 2'OH Group upon the Binding of Nucleotides by Basic Polyamino Acids" @default.
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- W1989285427 doi "https://doi.org/10.1111/j.1432-1033.1970.tb00927.x" @default.
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