Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989366952> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1989366952 abstract "We investigated the denaturation of tetrameric 20 beta-hydroxysteroid dehydrogenase (20R)-17 beta,20 beta,21-trihydroxysteroid:NAD+ oxidoreductase, EC 1.1.1.53) to find out whether intermediate states are formed during the process. The denaturation process was studied in the presence and absence of stabilizers, both specific, such as NADH, and non-specific, such as the salting-out anion phosphate. Changes in enzymatic activity, intrinsic protein fluorescence and far-ultraviolet circular dichroism were monitored. When NADH was present, denaturation of the enzyme by urea was a one-step transition between the native and the completely denatured state. In dilute phosphate, and even more so in concentrated phosphate, the existence of intermediate states with different stability is evidenced by the noncoincidence of the transition curves that probe for different functional and conformational aspects of the enzyme. Therefore, for 20 beta-hydroxysteroid dehydrogenase the formation of intermediates can be prevented by adding NADH, or enhanced by adding concentrated phosphate." @default.
- W1989366952 created "2016-06-24" @default.
- W1989366952 creator A5047773572 @default.
- W1989366952 creator A5052988170 @default.
- W1989366952 creator A5078893957 @default.
- W1989366952 creator A5080983305 @default.
- W1989366952 date "1987-08-01" @default.
- W1989366952 modified "2023-09-23" @default.
- W1989366952 title "Multiparameter study of denaturation of 20β-hydroxysteroid dehydrogenase by urea in the presence of stabilizing agents" @default.
- W1989366952 cites W1035449220 @default.
- W1989366952 cites W1519258948 @default.
- W1989366952 cites W1549635189 @default.
- W1989366952 cites W1971957066 @default.
- W1989366952 cites W1975728632 @default.
- W1989366952 cites W1976108782 @default.
- W1989366952 cites W1994421897 @default.
- W1989366952 cites W2023055140 @default.
- W1989366952 cites W2025213968 @default.
- W1989366952 cites W2047245131 @default.
- W1989366952 cites W2052069449 @default.
- W1989366952 cites W2052488365 @default.
- W1989366952 cites W2055446687 @default.
- W1989366952 cites W2061179481 @default.
- W1989366952 cites W2069136826 @default.
- W1989366952 cites W2070955753 @default.
- W1989366952 cites W2075584493 @default.
- W1989366952 cites W2079564988 @default.
- W1989366952 cites W2101494472 @default.
- W1989366952 doi "https://doi.org/10.1016/0167-4838(87)90054-9" @default.
- W1989366952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3475131" @default.
- W1989366952 hasPublicationYear "1987" @default.
- W1989366952 type Work @default.
- W1989366952 sameAs 1989366952 @default.
- W1989366952 citedByCount "5" @default.
- W1989366952 crossrefType "journal-article" @default.
- W1989366952 hasAuthorship W1989366952A5047773572 @default.
- W1989366952 hasAuthorship W1989366952A5052988170 @default.
- W1989366952 hasAuthorship W1989366952A5078893957 @default.
- W1989366952 hasAuthorship W1989366952A5080983305 @default.
- W1989366952 hasConcept C133571119 @default.
- W1989366952 hasConcept C13965031 @default.
- W1989366952 hasConcept C181199279 @default.
- W1989366952 hasConcept C185592680 @default.
- W1989366952 hasConcept C2776317432 @default.
- W1989366952 hasConcept C2776923230 @default.
- W1989366952 hasConcept C2779268744 @default.
- W1989366952 hasConcept C2779463914 @default.
- W1989366952 hasConcept C2780365088 @default.
- W1989366952 hasConcept C55493867 @default.
- W1989366952 hasConcept C71240020 @default.
- W1989366952 hasConcept C75520062 @default.
- W1989366952 hasConcept C8010536 @default.
- W1989366952 hasConceptScore W1989366952C133571119 @default.
- W1989366952 hasConceptScore W1989366952C13965031 @default.
- W1989366952 hasConceptScore W1989366952C181199279 @default.
- W1989366952 hasConceptScore W1989366952C185592680 @default.
- W1989366952 hasConceptScore W1989366952C2776317432 @default.
- W1989366952 hasConceptScore W1989366952C2776923230 @default.
- W1989366952 hasConceptScore W1989366952C2779268744 @default.
- W1989366952 hasConceptScore W1989366952C2779463914 @default.
- W1989366952 hasConceptScore W1989366952C2780365088 @default.
- W1989366952 hasConceptScore W1989366952C55493867 @default.
- W1989366952 hasConceptScore W1989366952C71240020 @default.
- W1989366952 hasConceptScore W1989366952C75520062 @default.
- W1989366952 hasConceptScore W1989366952C8010536 @default.
- W1989366952 hasLocation W19893669521 @default.
- W1989366952 hasLocation W19893669522 @default.
- W1989366952 hasOpenAccess W1989366952 @default.
- W1989366952 hasPrimaryLocation W19893669521 @default.
- W1989366952 hasRelatedWork W1656364975 @default.
- W1989366952 hasRelatedWork W1953994264 @default.
- W1989366952 hasRelatedWork W1986648910 @default.
- W1989366952 hasRelatedWork W1989604115 @default.
- W1989366952 hasRelatedWork W2000986031 @default.
- W1989366952 hasRelatedWork W2005305877 @default.
- W1989366952 hasRelatedWork W2030797369 @default.
- W1989366952 hasRelatedWork W2053834612 @default.
- W1989366952 hasRelatedWork W2087005413 @default.
- W1989366952 hasRelatedWork W2743382149 @default.
- W1989366952 isParatext "false" @default.
- W1989366952 isRetracted "false" @default.
- W1989366952 magId "1989366952" @default.
- W1989366952 workType "article" @default.