FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989379056> ?p ?o ?g. }

• W1989379056 endingPage "25180" @default.
• W1989379056 startingPage "25170" @default.
• W1989379056 abstract "We have developed a system to reconstitute all of the proposed steps of Okazaki fragment processing using purified yeast proteins and model substrates. DNA polymerase delta was shown to extend an upstream fragment to displace a downstream fragment into a flap. In most cases, the flap was removed by flap endonuclease 1 (FEN1), in a reaction required to remove initiator RNA in vivo. The nick left after flap removal could be sealed by DNA ligase I to complete fragment joining. An alternative pathway involving FEN1 and the nuclease/helicase Dna2 has been proposed for flaps that become long enough to bind replication protein A (RPA). RPA binding can inhibit FEN1, but Dna2 can shorten RPA-bound flaps so that RPA dissociates. Recent reconstitution results indicated that Pif1 helicase, a known component of fragment processing, accelerated flap displacement, allowing the inhibitory action of RPA. In results presented here, Pif1 promoted DNA polymerase delta to displace strands that achieve a length to bind RPA, but also to be Dna2 substrates. Significantly, RPA binding to long flaps inhibited the formation of the final ligation products in the reconstituted system without Dna2. However, Dna2 reversed that inhibition to restore efficient ligation. These results suggest that the two-nuclease pathway is employed in cells to process long flap intermediates promoted by Pif1." @default.
• W1989379056 created "2016-06-24" @default.
• W1989379056 creator A5000378293 @default.
• W1989379056 creator A5005315226 @default.
• W1989379056 creator A5032854120 @default.
• W1989379056 creator A5071638186 @default.
• W1989379056 date "2009-09-01" @default.
• W1989379056 modified "2023-10-12" @default.
• W1989379056 title "Pif1 Helicase Lengthens Some Okazaki Fragment Flaps Necessitating Dna2 Nuclease/Helicase Action in the Two-nuclease Processing Pathway" @default.
• W1989379056 cites W1729887059 @default.
• W1989379056 cites W1966219094 @default.
• W1989379056 cites W1966666560 @default.
• W1989379056 cites W1972746487 @default.
• W1989379056 cites W1973535211 @default.
• W1989379056 cites W1975098427 @default.
• W1989379056 cites W1988780940 @default.
• W1989379056 cites W1997002747 @default.
• W1989379056 cites W2003329456 @default.
• W1989379056 cites W2006220727 @default.
• W1989379056 cites W2010544809 @default.
• W1989379056 cites W2016218438 @default.
• W1989379056 cites W2018284593 @default.
• W1989379056 cites W2034535098 @default.
• W1989379056 cites W2040265375 @default.
• W1989379056 cites W2048897962 @default.
• W1989379056 cites W2053213954 @default.
• W1989379056 cites W2055566423 @default.
• W1989379056 cites W2055869110 @default.
• W1989379056 cites W2059184834 @default.
• W1989379056 cites W2062043277 @default.
• W1989379056 cites W2064264662 @default.
• W1989379056 cites W2076867773 @default.
• W1989379056 cites W2090110799 @default.
• W1989379056 cites W2095206600 @default.
• W1989379056 cites W2096549833 @default.
• W1989379056 cites W2100310707 @default.
• W1989379056 cites W2100626549 @default.
• W1989379056 cites W2111313204 @default.
• W1989379056 cites W2113176230 @default.
• W1989379056 cites W2135453449 @default.
• W1989379056 cites W2140230014 @default.
• W1989379056 cites W2146851796 @default.
• W1989379056 cites W2153262927 @default.
• W1989379056 cites W2153314555 @default.
• W1989379056 cites W2159127144 @default.
• W1989379056 cites W2161248815 @default.
• W1989379056 doi "https://doi.org/10.1074/jbc.m109.023325" @default.
• W1989379056 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2757220" @default.
• W1989379056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19605347" @default.
• W1989379056 hasPublicationYear "2009" @default.
• W1989379056 type Work @default.
• W1989379056 sameAs 1989379056 @default.
• W1989379056 citedByCount "90" @default.
• W1989379056 countsByYear W19893790562012 @default.
• W1989379056 countsByYear W19893790562013 @default.
• W1989379056 countsByYear W19893790562014 @default.
• W1989379056 countsByYear W19893790562015 @default.
• W1989379056 countsByYear W19893790562016 @default.
• W1989379056 countsByYear W19893790562017 @default.
• W1989379056 countsByYear W19893790562018 @default.
• W1989379056 countsByYear W19893790562019 @default.
• W1989379056 countsByYear W19893790562020 @default.
• W1989379056 countsByYear W19893790562021 @default.
• W1989379056 countsByYear W19893790562022 @default.
• W1989379056 countsByYear W19893790562023 @default.
• W1989379056 crossrefType "journal-article" @default.
• W1989379056 hasAuthorship W1989379056A5000378293 @default.
• W1989379056 hasAuthorship W1989379056A5005315226 @default.
• W1989379056 hasAuthorship W1989379056A5032854120 @default.
• W1989379056 hasAuthorship W1989379056A5071638186 @default.
• W1989379056 hasBestOaLocation W19893790561 @default.
• W1989379056 hasConcept C104317684 @default.
• W1989379056 hasConcept C11305352 @default.
• W1989379056 hasConcept C113271649 @default.
• W1989379056 hasConcept C153911025 @default.
• W1989379056 hasConcept C161223559 @default.
• W1989379056 hasConcept C185592680 @default.
• W1989379056 hasConcept C2756471 @default.
• W1989379056 hasConcept C2777028655 @default.
• W1989379056 hasConcept C2777271071 @default.
• W1989379056 hasConcept C552990157 @default.
• W1989379056 hasConcept C55493867 @default.
• W1989379056 hasConcept C67705224 @default.
• W1989379056 hasConcept C73573662 @default.
• W1989379056 hasConcept C82381507 @default.
• W1989379056 hasConcept C86339819 @default.
• W1989379056 hasConcept C86803240 @default.
• W1989379056 hasConcept C94966510 @default.
• W1989379056 hasConcept C95444343 @default.
• W1989379056 hasConcept C98086919 @default.
• W1989379056 hasConceptScore W1989379056C104317684 @default.
• W1989379056 hasConceptScore W1989379056C11305352 @default.
• W1989379056 hasConceptScore W1989379056C113271649 @default.
• W1989379056 hasConceptScore W1989379056C153911025 @default.
• W1989379056 hasConceptScore W1989379056C161223559 @default.
• W1989379056 hasConceptScore W1989379056C185592680 @default.
• W1989379056 hasConceptScore W1989379056C2756471 @default.
• W1989379056 hasConceptScore W1989379056C2777028655 @default.

• next