Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989396135> ?p ?o ?g. }
- W1989396135 endingPage "36" @default.
- W1989396135 startingPage "29" @default.
- W1989396135 abstract "The structural gene for glutathione S-transferase in Oryza sativa was successfully cloned from a cDNA library by the polymerase chain reaction method. The deduced amino acid sequence of this gene showed 44–66% similarity to the sequences of the class phi GSTs from Arabidopsis thaliana and Zea mays. This gene was expressed in Escherichia coli with the pET vector system and the gene product was purified to homogeneity by GSH–Sepharose affinity column chromatography. The expressed OsGSTF3-3 was a homo-dimer composed of 24 kDa subunit and its pI value was approximately 7.3. The OsGSTF3-3 was retained on GSH affinity column and its Km value for GSH was 0.28 mM. The OsGSTF3-3 displayed high activity toward 1-chloro-2,4-dinitrobenzene, a general GST substrate and also had high activities towards acetanilide herbicides, alachlor, and metolachlor. The OsGSTF3-3 was highly sensitive to inhibition by benastatin A and S-hexyl-GSH. From these results, the expressed OsGSTF3-3 is a phi class GST and seems to play an important role in the conjugation of the chloroacetanilide herbicides." @default.
- W1989396135 created "2016-06-24" @default.
- W1989396135 creator A5040422463 @default.
- W1989396135 creator A5042622579 @default.
- W1989396135 date "2005-09-01" @default.
- W1989396135 modified "2023-09-27" @default.
- W1989396135 title "Molecular cloning, expression, and characterization of a phi-type glutathione S-transferase from Oryza sativa" @default.
- W1989396135 cites W1519895850 @default.
- W1989396135 cites W1536923222 @default.
- W1989396135 cites W1546592314 @default.
- W1989396135 cites W1580238159 @default.
- W1989396135 cites W1591042311 @default.
- W1989396135 cites W1607888048 @default.
- W1989396135 cites W1974170352 @default.
- W1989396135 cites W2003836475 @default.
- W1989396135 cites W201844688 @default.
- W1989396135 cites W2025889416 @default.
- W1989396135 cites W2031030183 @default.
- W1989396135 cites W2033191938 @default.
- W1989396135 cites W2050442603 @default.
- W1989396135 cites W2055043387 @default.
- W1989396135 cites W2061247588 @default.
- W1989396135 cites W2073441900 @default.
- W1989396135 cites W2073670601 @default.
- W1989396135 cites W2074461690 @default.
- W1989396135 cites W2080242814 @default.
- W1989396135 cites W2088113363 @default.
- W1989396135 cites W2090208114 @default.
- W1989396135 cites W2094678368 @default.
- W1989396135 cites W2095068946 @default.
- W1989396135 cites W2097224528 @default.
- W1989396135 cites W2097320230 @default.
- W1989396135 cites W2101626712 @default.
- W1989396135 cites W2107957559 @default.
- W1989396135 cites W2128282994 @default.
- W1989396135 cites W2151293495 @default.
- W1989396135 cites W2733398579 @default.
- W1989396135 cites W2918031289 @default.
- W1989396135 cites W3026942661 @default.
- W1989396135 cites W4293247451 @default.
- W1989396135 doi "https://doi.org/10.1016/j.pestbp.2005.03.005" @default.
- W1989396135 hasPublicationYear "2005" @default.
- W1989396135 type Work @default.
- W1989396135 sameAs 1989396135 @default.
- W1989396135 citedByCount "27" @default.
- W1989396135 countsByYear W19893961352012 @default.
- W1989396135 countsByYear W19893961352013 @default.
- W1989396135 countsByYear W19893961352014 @default.
- W1989396135 countsByYear W19893961352015 @default.
- W1989396135 countsByYear W19893961352017 @default.
- W1989396135 countsByYear W19893961352018 @default.
- W1989396135 countsByYear W19893961352019 @default.
- W1989396135 countsByYear W19893961352020 @default.
- W1989396135 countsByYear W19893961352022 @default.
- W1989396135 countsByYear W19893961352023 @default.
- W1989396135 crossrefType "journal-article" @default.
- W1989396135 hasAuthorship W1989396135A5040422463 @default.
- W1989396135 hasAuthorship W1989396135A5042622579 @default.
- W1989396135 hasConcept C104317684 @default.
- W1989396135 hasConcept C153911025 @default.
- W1989396135 hasConcept C179247698 @default.
- W1989396135 hasConcept C181199279 @default.
- W1989396135 hasConcept C187882448 @default.
- W1989396135 hasConcept C19924922 @default.
- W1989396135 hasConcept C2776907368 @default.
- W1989396135 hasConcept C2778543611 @default.
- W1989396135 hasConcept C538909803 @default.
- W1989396135 hasConcept C55493867 @default.
- W1989396135 hasConcept C86803240 @default.
- W1989396135 hasConceptScore W1989396135C104317684 @default.
- W1989396135 hasConceptScore W1989396135C153911025 @default.
- W1989396135 hasConceptScore W1989396135C179247698 @default.
- W1989396135 hasConceptScore W1989396135C181199279 @default.
- W1989396135 hasConceptScore W1989396135C187882448 @default.
- W1989396135 hasConceptScore W1989396135C19924922 @default.
- W1989396135 hasConceptScore W1989396135C2776907368 @default.
- W1989396135 hasConceptScore W1989396135C2778543611 @default.
- W1989396135 hasConceptScore W1989396135C538909803 @default.
- W1989396135 hasConceptScore W1989396135C55493867 @default.
- W1989396135 hasConceptScore W1989396135C86803240 @default.
- W1989396135 hasIssue "1" @default.
- W1989396135 hasLocation W19893961351 @default.
- W1989396135 hasOpenAccess W1989396135 @default.
- W1989396135 hasPrimaryLocation W19893961351 @default.
- W1989396135 hasRelatedWork W1969691270 @default.
- W1989396135 hasRelatedWork W1987068646 @default.
- W1989396135 hasRelatedWork W1989391836 @default.
- W1989396135 hasRelatedWork W1997598952 @default.
- W1989396135 hasRelatedWork W1998493057 @default.
- W1989396135 hasRelatedWork W2074435004 @default.
- W1989396135 hasRelatedWork W2133501236 @default.
- W1989396135 hasRelatedWork W2370583535 @default.
- W1989396135 hasRelatedWork W2381640052 @default.
- W1989396135 hasRelatedWork W2391137290 @default.
- W1989396135 hasVolume "83" @default.
- W1989396135 isParatext "false" @default.
- W1989396135 isRetracted "false" @default.
- W1989396135 magId "1989396135" @default.