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• W1989401067 startingPage "10414" @default.
• W1989401067 abstract "Traceability is of particular importance for those persons who suffer allergy or intolerance to some food component(s) and need a strict avoidance of the allergenic food. In this paper, methodologies are described to fingerprint the presence of allergenic species such as carrot, tomato, and celery by DNA detection. Three DNA extraction methods were applied on vegetables and foods containing or not containing the allergens, and the results were compared and discussed. Fast SYBR Green DNA melting curve temperature analyses and duplex PCR assays with internal control have been developed for detection of these allergenic vegetables and have been tested on commercial foods. Spiking food experiments were also performed, assessing that limits of detection (LOD) of 1 mg/kg for carrot and tomato DNA and 10 mg/kg for celery DNA have been reached." @default.
• W1989401067 created "2016-06-24" @default.
• W1989401067 creator A5027752039 @default.
• W1989401067 creator A5038331339 @default.
• W1989401067 creator A5053454237 @default.
• W1989401067 date "2011-09-21" @default.
• W1989401067 modified "2023-09-25" @default.
• W1989401067 title "Comparison of DNA Extraction Methods and Development of Duplex PCR and Real-Time PCR To Detect Tomato, Carrot, and Celery in Food" @default.
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• W1989401067 doi "https://doi.org/10.1021/jf202382s" @default.
• W1989401067 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21894887" @default.
• W1989401067 hasPublicationYear "2011" @default.
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