FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989424614> ?p ?o ?g. }

• W1989424614 endingPage "469" @default.
• W1989424614 startingPage "461" @default.
• W1989424614 abstract "ObjectiveRecent improvements in human β-globin vector design have fueled interest in gene therapy approaches to the treatment of human thalassemia and sickle cell disease (SCD). The present study was undertaken to determine whether human β-globin mRNA and protein could be obtained in the erythroid progeny of more primitive human target cells transduced with a retrovirus containing murine stem cell virus long terminal repeats, a phosphoglycerate kinase promoter driving the expression of a green fluorescence protein (GFP) cDNA, and an anti-sickling β-globin (β87+) gene under the control of an HS2, HS3, HS4 enhancer cassette.Materials and MethodsA two-step pseudotyping strategy was devised to obtain useful preparations of this virus. Primitive cells present in normal human cord blood (CB) and adult SCD patients' blood samples were infected and the level of gene transfer (% GFP+ cells) and erythroid-specific β87+-globin expression assessed.ResultsAnalysis of the proportion of infected cells that became GFP+ showed that this virus transduced ∼50% of initial CD34+ CB and SCD cells and up to 23% of cells able to regenerate both lymphoid and myeloid cells in sublethally irradiated primary and secondary NOD/SCID mice. β87+-globin transcripts were readily detected in erythroblasts generated from primitive transduced CB cells and SCD progenitors. Evidence of β87+-derived protein in transduced CB cell-derived erythroblasts also was obtained.ConclusionThese findings demonstrate that retroviral vector-based gene transfer approaches can be used to achieve human β-globin protein expression in the erythroid progeny of transplantable human precursors." @default.
• W1989424614 created "2016-06-24" @default.
• W1989424614 creator A5006372952 @default.
• W1989424614 creator A5009814106 @default.
• W1989424614 creator A5013322327 @default.
• W1989424614 creator A5020780987 @default.
• W1989424614 creator A5020887225 @default.
• W1989424614 creator A5024703985 @default.
• W1989424614 creator A5036772739 @default.
• W1989424614 creator A5056789730 @default.
• W1989424614 date "2004-05-01" @default.
• W1989424614 modified "2023-10-17" @default.
• W1989424614 title "Expression of an anti-sickling β-globin in human erythroblasts derived from retrovirally transduced primitive normal and sickle cell disease hematopoietic cells" @default.
• W1989424614 cites W108816917 @default.
• W1989424614 cites W1499802238 @default.
• W1989424614 cites W1703848171 @default.
• W1989424614 cites W188488539 @default.
• W1989424614 cites W1964178121 @default.
• W1989424614 cites W1987037884 @default.
• W1989424614 cites W1987483457 @default.
• W1989424614 cites W1988614032 @default.
• W1989424614 cites W1997170085 @default.
• W1989424614 cites W2006672992 @default.
• W1989424614 cites W2016146452 @default.
• W1989424614 cites W2021200400 @default.
• W1989424614 cites W2025275264 @default.
• W1989424614 cites W2035444138 @default.
• W1989424614 cites W2052150955 @default.
• W1989424614 cites W2054993171 @default.
• W1989424614 cites W2077697398 @default.
• W1989424614 cites W2093750771 @default.
• W1989424614 cites W2102707713 @default.
• W1989424614 cites W2111444354 @default.
• W1989424614 cites W2270154356 @default.
• W1989424614 cites W4236058153 @default.
• W1989424614 cites W4238350555 @default.
• W1989424614 doi "https://doi.org/10.1016/j.exphem.2004.02.001" @default.
• W1989424614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15145214" @default.
• W1989424614 hasPublicationYear "2004" @default.
• W1989424614 type Work @default.
• W1989424614 sameAs 1989424614 @default.
• W1989424614 citedByCount "23" @default.
• W1989424614 countsByYear W19894246142012 @default.
• W1989424614 countsByYear W19894246142013 @default.
• W1989424614 countsByYear W19894246142015 @default.
• W1989424614 countsByYear W19894246142017 @default.
• W1989424614 crossrefType "journal-article" @default.
• W1989424614 hasAuthorship W1989424614A5006372952 @default.
• W1989424614 hasAuthorship W1989424614A5009814106 @default.
• W1989424614 hasAuthorship W1989424614A5013322327 @default.
• W1989424614 hasAuthorship W1989424614A5020780987 @default.
• W1989424614 hasAuthorship W1989424614A5020887225 @default.
• W1989424614 hasAuthorship W1989424614A5024703985 @default.
• W1989424614 hasAuthorship W1989424614A5036772739 @default.
• W1989424614 hasAuthorship W1989424614A5056789730 @default.
• W1989424614 hasBestOaLocation W19894246141 @default.
• W1989424614 hasConcept C10205521 @default.
• W1989424614 hasConcept C104317684 @default.
• W1989424614 hasConcept C109159458 @default.
• W1989424614 hasConcept C111599444 @default.
• W1989424614 hasConcept C111936080 @default.
• W1989424614 hasConcept C125996951 @default.
• W1989424614 hasConcept C1491633281 @default.
• W1989424614 hasConcept C150194340 @default.
• W1989424614 hasConcept C153911025 @default.
• W1989424614 hasConcept C159047783 @default.
• W1989424614 hasConcept C171122931 @default.
• W1989424614 hasConcept C172680121 @default.
• W1989424614 hasConcept C201750760 @default.
• W1989424614 hasConcept C203014093 @default.
• W1989424614 hasConcept C2776317666 @default.
• W1989424614 hasConcept C2779234561 @default.
• W1989424614 hasConcept C2779282312 @default.
• W1989424614 hasConcept C28328180 @default.
• W1989424614 hasConcept C32470452 @default.
• W1989424614 hasConcept C39981086 @default.
• W1989424614 hasConcept C40767141 @default.
• W1989424614 hasConcept C54355233 @default.
• W1989424614 hasConcept C86803240 @default.
• W1989424614 hasConcept C95444343 @default.
• W1989424614 hasConceptScore W1989424614C10205521 @default.
• W1989424614 hasConceptScore W1989424614C104317684 @default.
• W1989424614 hasConceptScore W1989424614C109159458 @default.
• W1989424614 hasConceptScore W1989424614C111599444 @default.
• W1989424614 hasConceptScore W1989424614C111936080 @default.
• W1989424614 hasConceptScore W1989424614C125996951 @default.
• W1989424614 hasConceptScore W1989424614C1491633281 @default.
• W1989424614 hasConceptScore W1989424614C150194340 @default.
• W1989424614 hasConceptScore W1989424614C153911025 @default.
• W1989424614 hasConceptScore W1989424614C159047783 @default.
• W1989424614 hasConceptScore W1989424614C171122931 @default.
• W1989424614 hasConceptScore W1989424614C172680121 @default.
• W1989424614 hasConceptScore W1989424614C201750760 @default.
• W1989424614 hasConceptScore W1989424614C203014093 @default.
• W1989424614 hasConceptScore W1989424614C2776317666 @default.
• W1989424614 hasConceptScore W1989424614C2779234561 @default.
• W1989424614 hasConceptScore W1989424614C2779282312 @default.
• W1989424614 hasConceptScore W1989424614C28328180 @default.

• next