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• W1989469421 abstract "Background: Decreased endothelial Nitric oxide (NO) bioavailability is one of the earliest events of endothelial dysfunction. Assessment of microvascular blood flow using a Laser Doppler Imager during local noninvasive administration of L-N-Arginine-Methyl-Ester (L-NAME) by skin iontophoresis may help discriminate the relative contributions of NO and non-NO pathways during a skin thermal hyperemic test. Methods: In healthy nonsmokers, the effects of thermal vasodilation and sodium nitroprusside–mediated vasodilation were tested on skin pretreated with 0.9% saline solution, 2% L-NAME iontophoresis (n = 12), or intradermal injection of 25 nmol L-NAME (n = 10). The effects of L-NAME iontophoresis were also measured in a group of smokers (n = 10). Results: L-NAME iontophoresis and intradermal injection of L-NAME decreased the skin response to local heating to a similar degree (−41% ± 4% vs. −44% ± 6%). L-NAME iontophoresis site-to-site and day-to-day coefficients of correlation were 0.83 and 0.76, respectively (P < 0.01). The site-to-site and day-to-day coefficients of correlation of L-NAME injection were lower than those of iontophoresis at 0.66 (P < 0.05) and 0.12, respectively (P = not significant). Sodium nitroprusside–induced skin hyperemia was not affected by L-NAME administration. L-NAME iontophoresis–mediated inhibition of skin thermal hyperemia was greater in smokers than in nonsmokers (P < 0.05). Conclusions: Laser Doppler Imager assessment of skin thermal hyperemia after L-NAME iontophoresis provides a reproducible and selective bedside method of qualitatively analyzing the contribution of the NO pathway to microvascular vasomotor function." @default.
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• W1989469421 date "2013-05-01" @default.
• W1989469421 modified "2023-09-24" @default.
• W1989469421 title "L-NAME Iontophoresis" @default.
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• W1989469421 doi "https://doi.org/10.1097/fjc.0b013e3182858f81" @default.
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