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- W1989528887 abstract "MALDI (matrix-assisted laser desorption/ionization) is one of the most important techniques used to produce large biomolecular ions in the gas phase. Surprisingly, the exact ionization mechanism is still not well understood and absolute values for the ion yields are scarce. This is in part due to the unknown efficiencies of typical detectors, especially for heavy biomolecular ions. As an alternative, charged particles can be non-destructively detected using an image-charge detector where the output voltage signal is proportional to the total charge within the device. In this paper, we report an absolute calibration which provides the voltage output per detected electronic charge in our experimental arrangement. A minimum of 3 × 103 ions were required to distinguish the signal above background noise in a single pass through the device, which could be further reduced using filtering techniques. The calibration results have been applied to raw MALDI spectra to measure absolute ion yields of both matrix and analyte ions." @default.
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- W1989528887 date "2010-03-09" @default.
- W1989528887 modified "2023-10-10" @default.
- W1989528887 title "Determination of absolute ion yields from a MALDI source through calibration of an image-charge detector" @default.
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- W1989528887 doi "https://doi.org/10.1088/0957-0233/21/4/045802" @default.
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