FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989531597> ?p ?o ?g. }

• W1989531597 endingPage "2734" @default.
• W1989531597 startingPage "2730" @default.
• W1989531597 abstract "It has been hypothesized that penicillin acts as a structural analog of the acyl-D-alanyl-D-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active site of the enzyme(s) that crosslinks the cell wall to form an inactive penicilloyl enzyme [Tipper, D.J. & Strominger, J.L. (1965) Proc. Natl. Acad. Sci. USA 64, 1133-1138]. This study directly proves that penicillin acylates the active site of two penicillin-sensitive enzymes, D-alanine carboxypeptidases from Bacillus stearothermophilus and Bacillus subtilis. Active site peptides were generated by chemical or enzymatic cleavage of these carboxypeptidases after covalently labeling with [14C]penicillin G or after trapping an acyl-enzyme intermediate derived from the depsipeptide substrate. [14C]diacetyl-L-lysyl-D-alanyl-D-lactate. The amino acid sequences of the penicillin- and substrate-labeled peptides were identical. Both penicillin and substrate were covalently bound via an ester linkage to the same active site residue, a serine at position 36 of the B. stearothermophilus carboxypeptidase and the corresponding serine in the B. subtilis carboxypeptidase. The two D-alanine carboxypeptidases showed significant homology around the active site. Moreover, homology between these two enzymes and four beta-lactamases of known sequence suggests that these two groups of enzymes are evolutionally related." @default.
• W1989531597 created "2016-06-24" @default.
• W1989531597 creator A5013759935 @default.
• W1989531597 creator A5025648724 @default.
• W1989531597 creator A5040129865 @default.
• W1989531597 creator A5044985980 @default.
• W1989531597 date "1979-06-01" @default.
• W1989531597 modified "2023-10-03" @default.
• W1989531597 title "Mechanism of penicillin action: penicillin and substrate bind covalently to the same active site serine in two bacterial D-alanine carboxypeptidases." @default.
• W1989531597 cites W12232001 @default.
• W1989531597 cites W1486894952 @default.
• W1989531597 cites W1539180171 @default.
• W1989531597 cites W1546150357 @default.
• W1989531597 cites W1550256141 @default.
• W1989531597 cites W1550519918 @default.
• W1989531597 cites W1554515894 @default.
• W1989531597 cites W1578348315 @default.
• W1989531597 cites W1678903690 @default.
• W1989531597 cites W1912943847 @default.
• W1989531597 cites W1970026389 @default.
• W1989531597 cites W1978959283 @default.
• W1989531597 cites W1984515631 @default.
• W1989531597 cites W1986924706 @default.
• W1989531597 cites W1987570261 @default.
• W1989531597 cites W2004228048 @default.
• W1989531597 cites W2004562533 @default.
• W1989531597 cites W2030302405 @default.
• W1989531597 cites W2042640333 @default.
• W1989531597 cites W2090536074 @default.
• W1989531597 cites W2092848100 @default.
• W1989531597 cites W2102843520 @default.
• W1989531597 cites W2278120600 @default.
• W1989531597 cites W2465977606 @default.
• W1989531597 cites W3119994227 @default.
• W1989531597 doi "https://doi.org/10.1073/pnas.76.6.2730" @default.
• W1989531597 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/383682" @default.
• W1989531597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/111240" @default.
• W1989531597 hasPublicationYear "1979" @default.
• W1989531597 type Work @default.
• W1989531597 sameAs 1989531597 @default.
• W1989531597 citedByCount "112" @default.
• W1989531597 countsByYear W19895315972013 @default.
• W1989531597 countsByYear W19895315972015 @default.
• W1989531597 countsByYear W19895315972016 @default.
• W1989531597 countsByYear W19895315972017 @default.
• W1989531597 countsByYear W19895315972018 @default.
• W1989531597 countsByYear W19895315972019 @default.
• W1989531597 countsByYear W19895315972020 @default.
• W1989531597 countsByYear W19895315972021 @default.
• W1989531597 countsByYear W19895315972022 @default.
• W1989531597 countsByYear W19895315972023 @default.
• W1989531597 crossrefType "journal-article" @default.
• W1989531597 hasAuthorship W1989531597A5013759935 @default.
• W1989531597 hasAuthorship W1989531597A5025648724 @default.
• W1989531597 hasAuthorship W1989531597A5040129865 @default.
• W1989531597 hasAuthorship W1989531597A5044985980 @default.
• W1989531597 hasBestOaLocation W19895315971 @default.
• W1989531597 hasConcept C108477463 @default.
• W1989531597 hasConcept C181199279 @default.
• W1989531597 hasConcept C185592680 @default.
• W1989531597 hasConcept C2776414213 @default.
• W1989531597 hasConcept C2777272437 @default.
• W1989531597 hasConcept C2779856020 @default.
• W1989531597 hasConcept C41183919 @default.
• W1989531597 hasConcept C515207424 @default.
• W1989531597 hasConcept C523546767 @default.
• W1989531597 hasConcept C54355233 @default.
• W1989531597 hasConcept C55493867 @default.
• W1989531597 hasConcept C71240020 @default.
• W1989531597 hasConcept C86803240 @default.
• W1989531597 hasConceptScore W1989531597C108477463 @default.
• W1989531597 hasConceptScore W1989531597C181199279 @default.
• W1989531597 hasConceptScore W1989531597C185592680 @default.
• W1989531597 hasConceptScore W1989531597C2776414213 @default.
• W1989531597 hasConceptScore W1989531597C2777272437 @default.
• W1989531597 hasConceptScore W1989531597C2779856020 @default.
• W1989531597 hasConceptScore W1989531597C41183919 @default.
• W1989531597 hasConceptScore W1989531597C515207424 @default.
• W1989531597 hasConceptScore W1989531597C523546767 @default.
• W1989531597 hasConceptScore W1989531597C54355233 @default.
• W1989531597 hasConceptScore W1989531597C55493867 @default.
• W1989531597 hasConceptScore W1989531597C71240020 @default.
• W1989531597 hasConceptScore W1989531597C86803240 @default.
• W1989531597 hasIssue "6" @default.
• W1989531597 hasLocation W19895315971 @default.
• W1989531597 hasLocation W19895315972 @default.
• W1989531597 hasLocation W19895315973 @default.
• W1989531597 hasLocation W19895315974 @default.
• W1989531597 hasOpenAccess W1989531597 @default.
• W1989531597 hasPrimaryLocation W19895315971 @default.
• W1989531597 hasRelatedWork W1500760553 @default.
• W1989531597 hasRelatedWork W1574264634 @default.
• W1989531597 hasRelatedWork W1644178486 @default.
• W1989531597 hasRelatedWork W1964729410 @default.
• W1989531597 hasRelatedWork W2006946696 @default.
• W1989531597 hasRelatedWork W2026586334 @default.
• W1989531597 hasRelatedWork W2040249061 @default.
• W1989531597 hasRelatedWork W2046782971 @default.

• next