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- W1989589336 abstract "Chromatographic separation of lithocholic acid from a mixture of four unconjugated bile acids was achieved in 5 sec. The system consisted of Gelman ITLC type SG chromatography sheets, Seprachrom miniature chromatography chambers, an isooctane—isopropyl ether—acetic acid (2:1:0.04, v/v/v) solvent system and a 5 % H2SO4 in methanol detection spray. The fluorescent spots were quantitated by direct scanning of the chromatograms with a Farrand Mark I spectrofluorometer. Maximum excitation and emission wavelengths were 375 and 436 nm, respectively. Accurate measurement of lithocholic acid was performed within the range of 25 to 175 ng." @default.
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- W1989589336 date "1979-01-01" @default.
- W1989589336 modified "2023-09-27" @default.
- W1989589336 title "Novel thin-layer chromatographic separation and spectro- flourometric quantitation of lithocholic acid" @default.
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- W1989589336 doi "https://doi.org/10.1016/0021-9673(79)80022-9" @default.
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