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• W1989590220 abstract "<p>Autoradiographic studies of ³H-uridine incorporation (20-min incubation) and dynamics of radioactive particle translocation from nucleolus into cytoplasm (following 80-min postincubation in non-radioactive medium) in root meristematic cells of soybean have been carried out. The experiment was performed with plants subjected to 4-day acclimation in chilling or subjected to 2-hour cold stress and control plants. Three cultivars of soybean: Mazowia, Polan and Progres (cultivated in Poland) were used in the experiment.</p><p>It has been shown that in control conditions the greatest number of RNA precursor is incorporated into nucleoli after 20-min incubation. Following 80-min postincubation cytoplasm is the most radioactive area of the cell - this mainly testifies to dynamic translocation of radioactive ribosome subunits from nucleolus into cytoplasm.</p><p>In chilling conditions the reduction of ³H-uridine incorporation into cells occurs, as compared to control conditions. Plants subjected to a 4-day acclimation incorporate the radioactive precursor more intensively than plants subjected to cold stress.</p><p>Following 80-min postincubation - in the case of acclimated plants - the nucleolus is the most radioactive area of the cell, which testifies to accumulation of pre-rRNA in it. After the cold stress cytoplasm is more radioactive than the nucleolus. In all three cultivars the processes of synthesis and transport of pre-rRNA particles are similar, only their intensity is different.</p><p>Morphometric measurements of nucleoli in all cultivars subjected to 4-day chilling have shown that root cell nucleoli are larger than those in control. This phenomenon can be connected with stronger inhibition of rRNA transport than its synthesis.</p>" @default.
• W1989590220 created "2016-06-24" @default.
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• W1989590220 date "2011-01-01" @default.
• W1989590220 modified "2023-10-01" @default.
• W1989590220 title "Effect of short- and long-lasting chilling on pre-rRNA synthesis and transport in root meristematic cells of three soybean cultivars" @default.
• W1989590220 doi "https://doi.org/10.5586/asbp.2003.041" @default.
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