FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989590902> ?p ?o ?g. }

• W1989590902 endingPage "4075" @default.
• W1989590902 startingPage "4066" @default.
• W1989590902 abstract "We have developed a simple method for reconstituting pure, integral membrane proteins into phospholipid-protein vesicles. The method does not depend on use of detergents or sonication. It has been used successfully with three different types of integral membrane proteins: UDPglucuronosyltransferase (EC 2.4.1.17) from pig liver microsomes, cytochrome oxidase (EC 1.9.3.1) from pig heart, and bacteriorhodopsin from Halobacterium halobium. The method depends on preparing unilamellar vesicles of dimyristoylphosphatidylcholine (DMPC) that contain a small amount of myristate as fusogen. Under conditions that the vesicles of DMPC have the property of fusing, all of the above proteins incorporated into bilayers. Two events appear to be involved in forming the phospholipid-protein complexes. The first is a rapid insertion of all proteins into a small percentage of total vesicles. The second is slower but continued fusion of the remaining phospholipid-protein vesicles, or proteoliposomes, with small unilamellar vesicles of DMPC. This latter process was inhibited by conditions under which vesicles of DMPC themselves would not fuse. On the basis of proton pumping by bacteriorhodopsin and negative staining, the vesicles were unilamellar and large. The data suggest that insertion of the above integral membrane proteins into vesicles occurred independently of fusion between vesicles." @default.
• W1989590902 created "2016-06-24" @default.
• W1989590902 creator A5046416564 @default.
• W1989590902 creator A5047775770 @default.
• W1989590902 date "1985-07-01" @default.
• W1989590902 modified "2023-10-02" @default.
• W1989590902 title "Reconstitution of membrane proteins. Spontaneous association of integral membrane proteins with preformed unilamellar lipid bilayers" @default.
• W1989590902 cites W1486318341 @default.
• W1989590902 cites W1501887526 @default.
• W1989590902 cites W1525291533 @default.
• W1989590902 cites W1525385499 @default.
• W1989590902 cites W1539854477 @default.
• W1989590902 cites W1548361323 @default.
• W1989590902 cites W1549075232 @default.
• W1989590902 cites W1550221585 @default.
• W1989590902 cites W1553938468 @default.
• W1989590902 cites W1559996241 @default.
• W1989590902 cites W1582082059 @default.
• W1989590902 cites W1594934722 @default.
• W1989590902 cites W1606935319 @default.
• W1989590902 cites W1887068553 @default.
• W1989590902 cites W1965486134 @default.
• W1989590902 cites W1973948888 @default.
• W1989590902 cites W1986410640 @default.
• W1989590902 cites W2000356911 @default.
• W1989590902 cites W2013587333 @default.
• W1989590902 cites W2020901657 @default.
• W1989590902 cites W2022496255 @default.
• W1989590902 cites W2024685716 @default.
• W1989590902 cites W2030360492 @default.
• W1989590902 cites W2033272827 @default.
• W1989590902 cites W2039545786 @default.
• W1989590902 cites W2043605564 @default.
• W1989590902 cites W2050292589 @default.
• W1989590902 cites W2054328585 @default.
• W1989590902 cites W2055836058 @default.
• W1989590902 cites W2063436389 @default.
• W1989590902 cites W2064282459 @default.
• W1989590902 cites W2067026318 @default.
• W1989590902 cites W2068968136 @default.
• W1989590902 cites W2077504813 @default.
• W1989590902 cites W2083050479 @default.
• W1989590902 cites W2083104119 @default.
• W1989590902 cites W2085511457 @default.
• W1989590902 cites W2102131116 @default.
• W1989590902 cites W2108857123 @default.
• W1989590902 cites W2146923243 @default.
• W1989590902 cites W2436880153 @default.
• W1989590902 cites W5096540 @default.
• W1989590902 cites W61388669 @default.
• W1989590902 doi "https://doi.org/10.1021/bi00336a040" @default.
• W1989590902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2996590" @default.
• W1989590902 hasPublicationYear "1985" @default.
• W1989590902 type Work @default.
• W1989590902 sameAs 1989590902 @default.
• W1989590902 citedByCount "44" @default.
• W1989590902 crossrefType "journal-article" @default.
• W1989590902 hasAuthorship W1989590902A5046416564 @default.
• W1989590902 hasAuthorship W1989590902A5047775770 @default.
• W1989590902 hasConcept C103038307 @default.
• W1989590902 hasConcept C12554922 @default.
• W1989590902 hasConcept C130316041 @default.
• W1989590902 hasConcept C144647389 @default.
• W1989590902 hasConcept C161200384 @default.
• W1989590902 hasConcept C185154212 @default.
• W1989590902 hasConcept C185592680 @default.
• W1989590902 hasConcept C189542057 @default.
• W1989590902 hasConcept C2778918659 @default.
• W1989590902 hasConcept C39944091 @default.
• W1989590902 hasConcept C41625074 @default.
• W1989590902 hasConcept C55493867 @default.
• W1989590902 hasConcept C86803240 @default.
• W1989590902 hasConceptScore W1989590902C103038307 @default.
• W1989590902 hasConceptScore W1989590902C12554922 @default.
• W1989590902 hasConceptScore W1989590902C130316041 @default.
• W1989590902 hasConceptScore W1989590902C144647389 @default.
• W1989590902 hasConceptScore W1989590902C161200384 @default.
• W1989590902 hasConceptScore W1989590902C185154212 @default.
• W1989590902 hasConceptScore W1989590902C185592680 @default.
• W1989590902 hasConceptScore W1989590902C189542057 @default.
• W1989590902 hasConceptScore W1989590902C2778918659 @default.
• W1989590902 hasConceptScore W1989590902C39944091 @default.
• W1989590902 hasConceptScore W1989590902C41625074 @default.
• W1989590902 hasConceptScore W1989590902C55493867 @default.
• W1989590902 hasConceptScore W1989590902C86803240 @default.
• W1989590902 hasIssue "15" @default.
• W1989590902 hasLocation W19895909021 @default.
• W1989590902 hasLocation W19895909022 @default.
• W1989590902 hasOpenAccess W1989590902 @default.
• W1989590902 hasPrimaryLocation W19895909021 @default.
• W1989590902 hasRelatedWork W1989176019 @default.
• W1989590902 hasRelatedWork W2008976522 @default.
• W1989590902 hasRelatedWork W2015799066 @default.
• W1989590902 hasRelatedWork W2024372093 @default.
• W1989590902 hasRelatedWork W2062717317 @default.
• W1989590902 hasRelatedWork W2122118872 @default.
• W1989590902 hasRelatedWork W2153425709 @default.
• W1989590902 hasRelatedWork W2395684028 @default.

• next