Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989638716> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1989638716 endingPage "346" @default.
- W1989638716 startingPage "341" @default.
- W1989638716 abstract "Cultured Chinese hamster ovary (CHO) cells suspended in their growth medium were forced by gas pressure through the uniformly sized micropores of filter membranes. This procedure caused transient damage to the plasma membrane, which increased the permeability of the cells to exogenous molecules. This filtroporation was indicated by uptake of fluorescent dextran molecules up to 500,000 MW in cells deemed viable by trypan blue dye exclusion. The macromolecular uptake was increased if the driving pressure was increased at constant micropore size, or if the micropore size was decreased at constant driving pressure. Larger membrane perturbations permitted uptake of a luciferase reporter plasmid, which resulted in transfection of the CHO cells with the surviving cells expressing luciferase activity after 2 days in culture. This simple and general new method of porating cells in suspension may be optimized to incorporate the desired macromolecules while retaining the maximum viability." @default.
- W1989638716 created "2016-06-24" @default.
- W1989638716 creator A5070992149 @default.
- W1989638716 creator A5081592170 @default.
- W1989638716 creator A5083643944 @default.
- W1989638716 date "1999-11-05" @default.
- W1989638716 modified "2023-10-17" @default.
- W1989638716 title "Filtroporation: A simple, reliable technique for transfection and macromolecular loading of cells in suspension" @default.
- W1989638716 cites W1556528051 @default.
- W1989638716 cites W1965372133 @default.
- W1989638716 cites W1970794086 @default.
- W1989638716 cites W1994945973 @default.
- W1989638716 cites W2001677044 @default.
- W1989638716 cites W2009628424 @default.
- W1989638716 cites W2015156947 @default.
- W1989638716 cites W2016456601 @default.
- W1989638716 cites W2026411959 @default.
- W1989638716 cites W2032463086 @default.
- W1989638716 cites W2041653279 @default.
- W1989638716 cites W2070289031 @default.
- W1989638716 cites W2082243712 @default.
- W1989638716 cites W2088432341 @default.
- W1989638716 cites W2109809176 @default.
- W1989638716 cites W2111593103 @default.
- W1989638716 cites W2133466768 @default.
- W1989638716 cites W2138766402 @default.
- W1989638716 cites W2138949734 @default.
- W1989638716 cites W2148694525 @default.
- W1989638716 cites W966437841 @default.
- W1989638716 doi "https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<341::aid-bit12>3.0.co;2-i" @default.
- W1989638716 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10486133" @default.
- W1989638716 hasPublicationYear "1999" @default.
- W1989638716 type Work @default.
- W1989638716 sameAs 1989638716 @default.
- W1989638716 citedByCount "9" @default.
- W1989638716 countsByYear W19896387162018 @default.
- W1989638716 countsByYear W19896387162021 @default.
- W1989638716 countsByYear W19896387162022 @default.
- W1989638716 countsByYear W19896387162023 @default.
- W1989638716 crossrefType "journal-article" @default.
- W1989638716 hasAuthorship W1989638716A5070992149 @default.
- W1989638716 hasAuthorship W1989638716A5081592170 @default.
- W1989638716 hasAuthorship W1989638716A5083643944 @default.
- W1989638716 hasConcept C104317684 @default.
- W1989638716 hasConcept C111335760 @default.
- W1989638716 hasConcept C12554922 @default.
- W1989638716 hasConcept C1491633281 @default.
- W1989638716 hasConcept C170493617 @default.
- W1989638716 hasConcept C175656101 @default.
- W1989638716 hasConcept C185592680 @default.
- W1989638716 hasConcept C204389451 @default.
- W1989638716 hasConcept C2777339698 @default.
- W1989638716 hasConcept C2780070996 @default.
- W1989638716 hasConcept C41625074 @default.
- W1989638716 hasConcept C43617362 @default.
- W1989638716 hasConcept C54009773 @default.
- W1989638716 hasConcept C55493867 @default.
- W1989638716 hasConcept C86803240 @default.
- W1989638716 hasConceptScore W1989638716C104317684 @default.
- W1989638716 hasConceptScore W1989638716C111335760 @default.
- W1989638716 hasConceptScore W1989638716C12554922 @default.
- W1989638716 hasConceptScore W1989638716C1491633281 @default.
- W1989638716 hasConceptScore W1989638716C170493617 @default.
- W1989638716 hasConceptScore W1989638716C175656101 @default.
- W1989638716 hasConceptScore W1989638716C185592680 @default.
- W1989638716 hasConceptScore W1989638716C204389451 @default.
- W1989638716 hasConceptScore W1989638716C2777339698 @default.
- W1989638716 hasConceptScore W1989638716C2780070996 @default.
- W1989638716 hasConceptScore W1989638716C41625074 @default.
- W1989638716 hasConceptScore W1989638716C43617362 @default.
- W1989638716 hasConceptScore W1989638716C54009773 @default.
- W1989638716 hasConceptScore W1989638716C55493867 @default.
- W1989638716 hasConceptScore W1989638716C86803240 @default.
- W1989638716 hasIssue "3" @default.
- W1989638716 hasLocation W19896387161 @default.
- W1989638716 hasLocation W19896387162 @default.
- W1989638716 hasOpenAccess W1989638716 @default.
- W1989638716 hasPrimaryLocation W19896387161 @default.
- W1989638716 hasRelatedWork W1966310096 @default.
- W1989638716 hasRelatedWork W1980792881 @default.
- W1989638716 hasRelatedWork W1989638716 @default.
- W1989638716 hasRelatedWork W2005437836 @default.
- W1989638716 hasRelatedWork W2106209844 @default.
- W1989638716 hasRelatedWork W2125915011 @default.
- W1989638716 hasRelatedWork W2259399507 @default.
- W1989638716 hasRelatedWork W2504214846 @default.
- W1989638716 hasRelatedWork W4220766107 @default.
- W1989638716 hasRelatedWork W4240342201 @default.
- W1989638716 hasVolume "65" @default.
- W1989638716 isParatext "false" @default.
- W1989638716 isRetracted "false" @default.
- W1989638716 magId "1989638716" @default.
- W1989638716 workType "article" @default.