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• W1989646060 abstract "Abstract The action of HgCl 2 on intracellular pH (pH i ) and H + excretion has been investigated in unfertilized and fertilized sea urchin eggs. The results show that HgCl 2 in the micromolar range stimulated acid release by increasing the Na + /H + antiporter activity in both unfertilized and fertilized eggs, altering pH i regulation and cell metabolism. However, the mechanism of HgCl 2 -induced acid excretion appeared to be different before and after fertilization. Furthermore, HgCl 2 induced cell alkalinisation in unfertilized eggs and cell acidification in fertilized eggs. The mechanism of HgCl 2 alteration of pH i and origin of excreted H + were studied. In unfertilized eggs, it is postulated that HgCl 2 stimulates the diacylglycerol pathway of the phosphatidyl-inositol cycle probably by acting on protein kinase C in a similar manner to the phorbol ester TPA, leading to activation of the Na + /H + exchange. In fertilized eggs, HgCl 2 induced H + excretion appeared to be dependent on an increase in Ca 2+ influx, which in turn may have been responsible for activating the Ca 2+ -dependent Na + /H + exchange. Furthermore, internal H + production was stimulated: the cyanide-sensitive H + source involved uncoupling of mitochondria, the cyanide-insensitive part of H + production likely resulted from action on acidic intracellular compartments, both events mediated by an HgCl 2 -induced Ca 2+ release." @default.
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• W1989646060 date "1993-08-01" @default.
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• W1989646060 title "Effects of HgCl2 on intracellular pH in sea urchin eggs: activation of H+ excretion and Na+/H+ exchange activity" @default.
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• W1989646060 doi "https://doi.org/10.1016/0166-445x(93)90029-z" @default.
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