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• W1989738351 abstract "Abstract Applying tissue engineering principles to design an auto‐secretory device is a potential solution for patients suffering loss of salivary gland function. However, the largest challenge in implementing this solution is the primary culture of human salivary gland cells, because the cells are highly differentiated and difficult to expand in vitro. This situation leads to the lack of reports on the in vitro cell biology and physiology of human salivary gland cells. This study used a low‐calcium culture system to selectively cultivate human parotid gland acinar (PGAC) cells from tissues with high purity in cell composition. This condition enables PGAC cells to continuously proliferate and retain the phenotypes of epithelial acinar cells to express secreting products (α‐amylase) and function‐related proteins (aquaporin‐3, aquaporin‐5, and ZO‐1). Notably, when the cells reached confluence, three‐dimensional (3D) cell aggregates were observed in crowded regions. These self‐formed cell spheres were termed post‐confluence structures (PCSs). Unexpectedly, despite being cultured in the same media, cells in PCSs exhibited higher expression levels and different expression patterns of function‐related proteins compared to the two‐dimensional (2D) cells. Translocation of aquoporin‐3 from cytosolic to alongside the cell boundaries, and of ZO‐1 molecules to the boundary of the PCSs were also observed. These observations suggest that when PGAC cells cultured on the 2D substrate would form PCSs without the help of 3D scaffolds and retain certain differentiation and polarity. This phenomenon implies that it is possible to introduce 2D substrates instead of 3D scaffolds into artificial salivary gland tissue engineering. J. Cell. Physiol. 226: 3076–3085, 2011. © 2011 Wiley‐Liss, Inc." @default.
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• W1989738351 date "2011-08-24" @default.
• W1989738351 modified "2023-10-12" @default.
• W1989738351 title "Human salivary gland acinar cells spontaneously form three-dimensional structures and change the protein expression patterns" @default.
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• W1989738351 doi "https://doi.org/10.1002/jcp.22664" @default.
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