FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989763219> ?p ?o ?g. }

• W1989763219 endingPage "299" @default.
• W1989763219 startingPage "289" @default.
• W1989763219 abstract "MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 124:289-299 (1995) - doi:10.3354/meps124289 Simple procedure for simultaneous recovery of dissolved inorganic and organic nitrogen in 15N-tracer experiments and improving the isotopic mass balance Slawyk G, Raimbault P We developed a simple and reliable method which allows simultaneous isotope-ratio analysis of inorganic (DIN) and organic (DON) forms of nitrogen extracted from seawater. All forms of nitrogen under analysis are converted to ammonium, by diffusion with magnesium oxide, prior to collection on glass-fiber filters appropriate for mass spectrometric assay of 15N. Oxidized DIN forms (nitrate, nitrite) are reduced to ammonium in the presence of Devarda alloy. Conversion of DON to ammonium is performed by wet oxidation using potassium persulfate and subsequent reduction of the nitrate formed. Recovery tests, both for total nitrogen and 15N content, showed that this procedure is suitable for application in DI15N-isotope dilution experiments and DON-release studies. Recovery of total nitrogen from DIN and DON was nearly complete (94 to 97%). The variability in the experimental determination of 15N abundance was &LT2% and &LT4% for DIN and DON, respectively. We used the method to balance the 15N budget in nitrate and ammonium uptake experiments conducted in an oligotrophic area (tropical North Atlantic) by including, in addition to the substrate (DIN) and biomass (PON) pool, the DON pool. However, the use of glass-fiber filters (GF/F) for the collection of particulate matter produced a significant artifact, i.e. a large amount of small particles (&LT0.7 um, PON&LTGF/F; prochlorophytes and/or bacteria) passed through these filters and were recovered together with the DON in a combined pool. While inclusion of this combined pool led virtually to a complete accounting for the 15N label (99%) in all samples for nitrate uptake and in those for ammonium uptake incubated for &LT8 h, no mass balance was achieved during ammonium uptake lasting 10 to 24 h. We suggest that the 15N that was still missing (13%) resulted mainly from bottle containment effects such as ammonium-ion adsorption and/or PON adherence onto incubation bottle walls. Transfer of 15N label to the combined pool (nitrate experiment) and to the DON and PON&LTGF/F pools (ammonium experiment) represented up to 41, 38 and 20% of the total 15N taken up as DIN, respectively, and depended strongly upon the length of incubation. Failure to take these pathways of the missing 15N into account during traditional 15N uptake experiments involves risk of substantially underestimating new and regenerated production, at least in oligotrophic areas. The latter fact has considerable significance in the design of future 15N tracer methodologies. Organic nitrogen . Inorganic nitrogen . 15N methodology . Nitrogen uptake . 15N partitioning . Oceanic waters Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 124. Publication date: August 10, 1995 Print ISSN:0171-8630; Online ISSN:1616-1599 Copyright © 1995 Inter-Research." @default.
• W1989763219 created "2016-06-24" @default.
• W1989763219 creator A5027608231 @default.
• W1989763219 creator A5074039190 @default.
• W1989763219 date "1995-01-01" @default.
• W1989763219 modified "2023-09-27" @default.
• W1989763219 title "Simple procedure for simultaneous recovery of dissolved inorganic and organic nitrogen in 15N-tracer experiments and improving the isotopic mass balance" @default.
• W1989763219 doi "https://doi.org/10.3354/meps124289" @default.
• W1989763219 hasPublicationYear "1995" @default.
• W1989763219 type Work @default.
• W1989763219 sameAs 1989763219 @default.
• W1989763219 citedByCount "77" @default.
• W1989763219 countsByYear W19897632192012 @default.
• W1989763219 countsByYear W19897632192013 @default.
• W1989763219 countsByYear W19897632192014 @default.
• W1989763219 countsByYear W19897632192015 @default.
• W1989763219 countsByYear W19897632192016 @default.
• W1989763219 countsByYear W19897632192017 @default.
• W1989763219 countsByYear W19897632192018 @default.
• W1989763219 countsByYear W19897632192019 @default.
• W1989763219 countsByYear W19897632192020 @default.
• W1989763219 countsByYear W19897632192021 @default.
• W1989763219 countsByYear W19897632192022 @default.
• W1989763219 crossrefType "journal-article" @default.
• W1989763219 hasAuthorship W1989763219A5027608231 @default.
• W1989763219 hasAuthorship W1989763219A5074039190 @default.
• W1989763219 hasBestOaLocation W19897632191 @default.
• W1989763219 hasConcept C107872376 @default.
• W1989763219 hasConcept C121332964 @default.
• W1989763219 hasConcept C178790620 @default.
• W1989763219 hasConcept C185544564 @default.
• W1989763219 hasConcept C185592680 @default.
• W1989763219 hasConcept C2776384668 @default.
• W1989763219 hasConcept C2778863792 @default.
• W1989763219 hasConcept C2779870022 @default.
• W1989763219 hasConcept C36574619 @default.
• W1989763219 hasConcept C537208039 @default.
• W1989763219 hasConcept C8101879 @default.
• W1989763219 hasConcept C99649442 @default.
• W1989763219 hasConceptScore W1989763219C107872376 @default.
• W1989763219 hasConceptScore W1989763219C121332964 @default.
• W1989763219 hasConceptScore W1989763219C178790620 @default.
• W1989763219 hasConceptScore W1989763219C185544564 @default.
• W1989763219 hasConceptScore W1989763219C185592680 @default.
• W1989763219 hasConceptScore W1989763219C2776384668 @default.
• W1989763219 hasConceptScore W1989763219C2778863792 @default.
• W1989763219 hasConceptScore W1989763219C2779870022 @default.
• W1989763219 hasConceptScore W1989763219C36574619 @default.
• W1989763219 hasConceptScore W1989763219C537208039 @default.
• W1989763219 hasConceptScore W1989763219C8101879 @default.
• W1989763219 hasConceptScore W1989763219C99649442 @default.
• W1989763219 hasLocation W19897632191 @default.
• W1989763219 hasOpenAccess W1989763219 @default.
• W1989763219 hasPrimaryLocation W19897632191 @default.
• W1989763219 hasRelatedWork W1974073500 @default.
• W1989763219 hasRelatedWork W2007493442 @default.
• W1989763219 hasRelatedWork W2017418032 @default.
• W1989763219 hasRelatedWork W2020126175 @default.
• W1989763219 hasRelatedWork W2055285323 @default.
• W1989763219 hasRelatedWork W2087470741 @default.
• W1989763219 hasRelatedWork W2106116965 @default.
• W1989763219 hasRelatedWork W2131045070 @default.
• W1989763219 hasRelatedWork W2139794576 @default.
• W1989763219 hasRelatedWork W2460716337 @default.
• W1989763219 hasVolume "124" @default.
• W1989763219 isParatext "false" @default.
• W1989763219 isRetracted "false" @default.
• W1989763219 magId "1989763219" @default.
• W1989763219 workType "article" @default.