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- W1989813215 abstract "The peptide mastoparan 7 (MST7) stimulated ATP release in human erythrocytes. We explored intra- and extracellular processes governing the time-dependent accumulation of extracellular ATP (i.e., ATPe kinetics).Human erythrocytes were treated with MST7 in the presence or absence of two blockers of pannexin 1. ATPe concentration was monitored by luciferin-luciferase based real-time luminometry.Exposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling. However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe accumulation was inhibited by 49%. Erythrocytes pre-exposure to 10μM of either carbenoxolone or probenecid, two blockers of pannexin 1, exhibited a partial reduction of ATP efflux. Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice erythrocytes exposed to pannexin 1 blockers.MST7 induced release of ATP required either cell adhesion or strong activation of cAMP synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being fully blocked by pannexin 1 blockers.Kinetic analysis of extracellular ATP accumulation from human erythrocytes and potential effects on microcirculation." @default.
- W1989813215 created "2016-06-24" @default.
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- W1989813215 date "2013-10-01" @default.
- W1989813215 modified "2023-10-09" @default.
- W1989813215 title "Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes" @default.
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- W1989813215 doi "https://doi.org/10.1016/j.bbagen.2013.05.033" @default.
- W1989813215 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3999873" @default.
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