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• W1989909868 abstract "To replace damaged or diseased tissues, large tissue‐engineered constructs can be prepared by assembling modular components in a bottom‐up approach. However, a high‐speed method is needed to produce sufficient numbers of these modules for full‐sized tissue substitutes. To this end, a novel production technique is devised, combining air shearing and a plug flow reactor‐style design to rapidly produce large quantities of hydrogel‐based (here type I collagen) cylindrical modular components with tunable diameters and length. Using this technique, modules containing NIH 3T3 cells show greater than 95% viability while endothelial cell surface attachment and confluent monolayer formation are demonstrated. Additionally, the rapidly produced modules are used to assemble large tissue constructs (>1 cm 3 ) in vitro. Module building blocks containing luciferase‐expressing L929 cells are packed in full size adult rat‐liver‐shaped bioreactors and perfused with cell medium, to demonstrate the capacity to build organ‐shaped constructs; bioluminescence demonstrates sustained viability over 3 d. Cardiomyocyte‐embedded modules are also used to assemble electrically stimulatable contractile tissue." @default.
• W1989909868 created "2016-06-24" @default.
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• W1989909868 date "2014-06-03" @default.
• W1989909868 modified "2023-10-16" @default.
• W1989909868 title "A Novel High-Speed Production Process to Create Modular Components for the Bottom-Up Assembly of Large-Scale Tissue-Engineered Constructs" @default.
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• W1989909868 doi "https://doi.org/10.1002/adhm.201400150" @default.
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