Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989957696> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1989957696 endingPage "757" @default.
- W1989957696 startingPage "754" @default.
- W1989957696 abstract "Plant cell-wall polysaccharides offer an abundant energy source utilized by many microorganisms, thus playing a central role in carbon recycling. Aerobic microorganisms secrete carbohydrate-active enzymes (CAZymes) that catabolize this composite structure, comprising cellulose, hemicellulose and lignin, into simple compounds such as glucose. Carbohydrate-binding modules (CBMs) enhance the efficacy of associated CAZYmes. They are organized into families based on primary-sequence homology. CBM family 46 contains more than 40 different members, but has yet to be fully characterized. Here, a recombinant derivative of the C-terminal family 46 CBM module ( Bh CBM46) of Bacillus halodurans endo-β-1,4-glucanase B (CelB) was overexpressed in Escherichia coli and purified by immobilized metal-ion affinity chromatography. Preliminary structural characterization was carried out on Bh CBM46 crystallized in different conditions. The crystals of Bh CBM46 belonged to the tetragonal space group I 4 1 22. Data were collected for the native form and a selenomethionine derivative to 2.46 and 2.3 Å resolution, respectively. The Bh CBM46 structure was determined by a single-wavelength anomalous dispersion experiment using AutoSol from the PHENIX suite." @default.
- W1989957696 created "2016-06-24" @default.
- W1989957696 creator A5004641561 @default.
- W1989957696 creator A5019216535 @default.
- W1989957696 creator A5021225439 @default.
- W1989957696 creator A5037099802 @default.
- W1989957696 creator A5069105565 @default.
- W1989957696 creator A5091577197 @default.
- W1989957696 date "2014-05-10" @default.
- W1989957696 modified "2023-09-24" @default.
- W1989957696 title "Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-β-1,4-glucanase B (CelB) from<i>Bacillus halodurans</i>" @default.
- W1989957696 cites W1966408497 @default.
- W1989957696 cites W1988966516 @default.
- W1989957696 cites W2014694459 @default.
- W1989957696 cites W2043245651 @default.
- W1989957696 cites W2103714813 @default.
- W1989957696 cites W2108921801 @default.
- W1989957696 cites W2122559203 @default.
- W1989957696 cites W2122994276 @default.
- W1989957696 cites W2127130852 @default.
- W1989957696 cites W2129809239 @default.
- W1989957696 cites W2134465386 @default.
- W1989957696 cites W2147949826 @default.
- W1989957696 cites W2158355883 @default.
- W1989957696 cites W2163341755 @default.
- W1989957696 cites W2180229411 @default.
- W1989957696 doi "https://doi.org/10.1107/s2053230x14008395" @default.
- W1989957696 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4051530" @default.
- W1989957696 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24915086" @default.
- W1989957696 hasPublicationYear "2014" @default.
- W1989957696 type Work @default.
- W1989957696 sameAs 1989957696 @default.
- W1989957696 citedByCount "4" @default.
- W1989957696 countsByYear W19899576962014 @default.
- W1989957696 countsByYear W19899576962015 @default.
- W1989957696 crossrefType "journal-article" @default.
- W1989957696 hasAuthorship W1989957696A5004641561 @default.
- W1989957696 hasAuthorship W1989957696A5019216535 @default.
- W1989957696 hasAuthorship W1989957696A5021225439 @default.
- W1989957696 hasAuthorship W1989957696A5037099802 @default.
- W1989957696 hasAuthorship W1989957696A5069105565 @default.
- W1989957696 hasAuthorship W1989957696A5091577197 @default.
- W1989957696 hasBestOaLocation W19899576962 @default.
- W1989957696 hasConcept C104317684 @default.
- W1989957696 hasConcept C141603559 @default.
- W1989957696 hasConcept C167625842 @default.
- W1989957696 hasConcept C181199279 @default.
- W1989957696 hasConcept C185592680 @default.
- W1989957696 hasConcept C2776073808 @default.
- W1989957696 hasConcept C2776519085 @default.
- W1989957696 hasConcept C2778977261 @default.
- W1989957696 hasConcept C2779251873 @default.
- W1989957696 hasConcept C2779530776 @default.
- W1989957696 hasConcept C40767141 @default.
- W1989957696 hasConcept C547475151 @default.
- W1989957696 hasConcept C55493867 @default.
- W1989957696 hasConcept C86803240 @default.
- W1989957696 hasConcept C89423630 @default.
- W1989957696 hasConcept C92825506 @default.
- W1989957696 hasConceptScore W1989957696C104317684 @default.
- W1989957696 hasConceptScore W1989957696C141603559 @default.
- W1989957696 hasConceptScore W1989957696C167625842 @default.
- W1989957696 hasConceptScore W1989957696C181199279 @default.
- W1989957696 hasConceptScore W1989957696C185592680 @default.
- W1989957696 hasConceptScore W1989957696C2776073808 @default.
- W1989957696 hasConceptScore W1989957696C2776519085 @default.
- W1989957696 hasConceptScore W1989957696C2778977261 @default.
- W1989957696 hasConceptScore W1989957696C2779251873 @default.
- W1989957696 hasConceptScore W1989957696C2779530776 @default.
- W1989957696 hasConceptScore W1989957696C40767141 @default.
- W1989957696 hasConceptScore W1989957696C547475151 @default.
- W1989957696 hasConceptScore W1989957696C55493867 @default.
- W1989957696 hasConceptScore W1989957696C86803240 @default.
- W1989957696 hasConceptScore W1989957696C89423630 @default.
- W1989957696 hasConceptScore W1989957696C92825506 @default.
- W1989957696 hasIssue "6" @default.
- W1989957696 hasLocation W19899576961 @default.
- W1989957696 hasLocation W19899576962 @default.
- W1989957696 hasLocation W19899576963 @default.
- W1989957696 hasLocation W19899576964 @default.
- W1989957696 hasOpenAccess W1989957696 @default.
- W1989957696 hasPrimaryLocation W19899576961 @default.
- W1989957696 hasRelatedWork W1965423702 @default.
- W1989957696 hasRelatedWork W2016628627 @default.
- W1989957696 hasRelatedWork W2041249881 @default.
- W1989957696 hasRelatedWork W2072616837 @default.
- W1989957696 hasRelatedWork W2083264329 @default.
- W1989957696 hasRelatedWork W2097879647 @default.
- W1989957696 hasRelatedWork W2117726594 @default.
- W1989957696 hasRelatedWork W2360745033 @default.
- W1989957696 hasRelatedWork W2416836049 @default.
- W1989957696 hasRelatedWork W2990048777 @default.
- W1989957696 hasVolume "70" @default.
- W1989957696 isParatext "false" @default.
- W1989957696 isRetracted "false" @default.
- W1989957696 magId "1989957696" @default.
- W1989957696 workType "article" @default.