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• W1989968124 abstract "We report the purification of an ATP-dependent nucleosome remodeling factor (NURF) from Drosophila embryo extracts. NURF is composed of at least four polypeptides that act in concert with the GAGA transcription factor to alter chromatin structure at the hsp70 promoter. The energy requirement is attributed to an ATPase activity that is stimulated by nucleosomes but not by free DNA or histones, suggesting that NURF acts directly on a nucleosome to perturb its structure. This finding and the physical properties of NURF contrast sharply with the multisubunit SWI2/SNF2 complex, which has also been shown to alter nucleosomes in an ATP-dependent manner. The results suggest that two distinct systems may be involved in remodeling chromatin for transcription." @default.
• W1989968124 created "2016-06-24" @default.
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• W1989968124 date "1995-12-01" @default.
• W1989968124 modified "2023-10-18" @default.
• W1989968124 title "Purification and properties of an ATP-dependent nucleosome remodeling factor" @default.
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• W1989968124 doi "https://doi.org/10.1016/0092-8674(95)90216-3" @default.
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