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- W1989980147 abstract "Phosphodiesterases (PDE) are enzymes that catalyze the hydrolysis of cAMP/cGMP to 5′-AMP/GMP. In vitro assays have routinely assayed cAMP/cGMP levels as a direct indicator of PDE activity. Earlier PDE assays depended on radiometric detection of radiolabeled cAMP. Of late, nonradiometric cAMP detection systems have been developed that are cheaper and more amenable to high-throughput screening. Two such assays, namely the enzyme fragment complementation technology and homogeneous time-resolved fluorescence assays, are currently used for monitoring cAMP as a correlate for G-protein-coupled-receptor-induced cellular signaling events. Here, we have compared and validated both of these assays for the measurement of PDE4 enzyme activity in cell-free systems." @default.
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- W1989980147 date "2010-01-01" @default.
- W1989980147 modified "2023-09-24" @default.
- W1989980147 title "Evaluation of Nonradioactive Cell-Free cAMP Assays for Measuring in vitro Phosphodiesterase Activity" @default.
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- W1989980147 doi "https://doi.org/10.1159/000290641" @default.
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