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- W1990006536 abstract "Protein A of Staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. The same Protein A-peroxidase conjugate was used with antisera from five different species. Using this indirect test, membrane markers for T and B lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. Viral antigens on cells infected with measles, vesicular stomatitis, herpes or visna virus, respectively, were also stained in the protein A-peroxidase indirect test with a greater specificity than indirect method using anti-immunoglobulin. Paired preparations were examined in the light and electron microscope. Ultrastructural analysis showed that the protein A-peroxidase conjugate penetrated well through fixed viral membranes and resulted in fine resolution of antigenic sites." @default.
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- W1990006536 date "1977-11-01" @default.
- W1990006536 modified "2023-09-27" @default.
- W1990006536 title "Protein A-peroxidase: a valluable tool for the localization of antigens." @default.
- W1990006536 doi "https://doi.org/10.1177/25.11.199666" @default.
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