FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990014307> ?p ?o ?g. }

• W1990014307 abstract "The arsenal of fluorescent probes tailored to functional imaging of cells is rapidly growing and benefits from recent developments in imaging strategies. We here present two new molecular rotors characterized by a push-pull hyperconjugated structure and which absorb in the blue-green region of the spectrum. These probes display significant emission and lifetime increase by increasing local microviscosity. Owing to its concentration-independent nature, fluorescence lifetime is particularly suitable to report on environmental properties, such as viscosity, in the cellular context, i.e. where the fluorescent probe concentration cannot be controlled. Remarkably, we demonstrate that our probes allow for efficient intracellular viscosity measurements by means of the phasor approach to fluorescence lifetime imaging, a fit-free method that overcome the main drawbacks of conventional lifetime imaging (1). More specifically, we show that one probe can be used to monitor membrane organization (i.e. the fractions of liquid ordered and disordered phases) and membrane viscosity, whereas the other reports on the local viscosity in plasma membrane, lysosomes, and mitochondria (2). Notably, measurements were carried out in both physiological and non-physiological conditions, in view of correlating dysfunctional states of the cell with viscosity changes in intracellular organelles.1. Digman, M.A., Caiolfa, V.R., Zamai, M., and Gratton, E. (2008) Biophys J 94, L14-16.2. Battisti, A., Panettieri, S., Abbandonato, G., Jacchetti, E., Cardarelli, F., Signore, G., Beltram, F., and Bizzarri, R. (2013) Anal. Bioanal. Chem. 405, 6223-6233." @default.
• W1990014307 created "2016-06-24" @default.
• W1990014307 creator A5019956983 @default.
• W1990014307 creator A5033342633 @default.
• W1990014307 creator A5033478201 @default.
• W1990014307 creator A5039230712 @default.
• W1990014307 creator A5045396260 @default.
• W1990014307 creator A5060940793 @default.
• W1990014307 creator A5073809598 @default.
• W1990014307 creator A5075276042 @default.
• W1990014307 creator A5079885219 @default.
• W1990014307 date "2014-01-01" @default.
• W1990014307 modified "2023-10-02" @default.
• W1990014307 title "Imaging of Intracellular Viscosity and Membrane Order by New Molecular Rotors Suitable for Phasor Analysis of Fluorescence Lifetime" @default.
• W1990014307 doi "https://doi.org/10.1016/j.bpj.2013.11.188" @default.
• W1990014307 hasPublicationYear "2014" @default.
• W1990014307 type Work @default.
• W1990014307 sameAs 1990014307 @default.
• W1990014307 citedByCount "1" @default.
• W1990014307 countsByYear W19900143072019 @default.
• W1990014307 crossrefType "journal-article" @default.
• W1990014307 hasAuthorship W1990014307A5019956983 @default.
• W1990014307 hasAuthorship W1990014307A5033342633 @default.
• W1990014307 hasAuthorship W1990014307A5033478201 @default.
• W1990014307 hasAuthorship W1990014307A5039230712 @default.
• W1990014307 hasAuthorship W1990014307A5045396260 @default.
• W1990014307 hasAuthorship W1990014307A5060940793 @default.
• W1990014307 hasAuthorship W1990014307A5073809598 @default.
• W1990014307 hasAuthorship W1990014307A5075276042 @default.
• W1990014307 hasAuthorship W1990014307A5079885219 @default.
• W1990014307 hasBestOaLocation W19900143071 @default.
• W1990014307 hasConcept C113196181 @default.
• W1990014307 hasConcept C120665830 @default.
• W1990014307 hasConcept C121332964 @default.
• W1990014307 hasConcept C12554922 @default.
• W1990014307 hasConcept C127172972 @default.
• W1990014307 hasConcept C139932579 @default.
• W1990014307 hasConcept C151730666 @default.
• W1990014307 hasConcept C159467904 @default.
• W1990014307 hasConcept C159985019 @default.
• W1990014307 hasConcept C163258240 @default.
• W1990014307 hasConcept C176605952 @default.
• W1990014307 hasConcept C185592680 @default.
• W1990014307 hasConcept C192562407 @default.
• W1990014307 hasConcept C2779343474 @default.
• W1990014307 hasConcept C41625074 @default.
• W1990014307 hasConcept C43617362 @default.
• W1990014307 hasConcept C55493867 @default.
• W1990014307 hasConcept C57477423 @default.
• W1990014307 hasConcept C79879829 @default.
• W1990014307 hasConcept C86803240 @default.
• W1990014307 hasConcept C89227174 @default.
• W1990014307 hasConcept C91881484 @default.
• W1990014307 hasConcept C97355855 @default.
• W1990014307 hasConceptScore W1990014307C113196181 @default.
• W1990014307 hasConceptScore W1990014307C120665830 @default.
• W1990014307 hasConceptScore W1990014307C121332964 @default.
• W1990014307 hasConceptScore W1990014307C12554922 @default.
• W1990014307 hasConceptScore W1990014307C127172972 @default.
• W1990014307 hasConceptScore W1990014307C139932579 @default.
• W1990014307 hasConceptScore W1990014307C151730666 @default.
• W1990014307 hasConceptScore W1990014307C159467904 @default.
• W1990014307 hasConceptScore W1990014307C159985019 @default.
• W1990014307 hasConceptScore W1990014307C163258240 @default.
• W1990014307 hasConceptScore W1990014307C176605952 @default.
• W1990014307 hasConceptScore W1990014307C185592680 @default.
• W1990014307 hasConceptScore W1990014307C192562407 @default.
• W1990014307 hasConceptScore W1990014307C2779343474 @default.
• W1990014307 hasConceptScore W1990014307C41625074 @default.
• W1990014307 hasConceptScore W1990014307C43617362 @default.
• W1990014307 hasConceptScore W1990014307C55493867 @default.
• W1990014307 hasConceptScore W1990014307C57477423 @default.
• W1990014307 hasConceptScore W1990014307C79879829 @default.
• W1990014307 hasConceptScore W1990014307C86803240 @default.
• W1990014307 hasConceptScore W1990014307C89227174 @default.
• W1990014307 hasConceptScore W1990014307C91881484 @default.
• W1990014307 hasConceptScore W1990014307C97355855 @default.
• W1990014307 hasLocation W19900143071 @default.
• W1990014307 hasOpenAccess W1990014307 @default.
• W1990014307 hasPrimaryLocation W19900143071 @default.
• W1990014307 hasRelatedWork W1004784976 @default.
• W1990014307 hasRelatedWork W1839112063 @default.
• W1990014307 hasRelatedWork W1971873039 @default.
• W1990014307 hasRelatedWork W1997940024 @default.
• W1990014307 hasRelatedWork W2004524284 @default.
• W1990014307 hasRelatedWork W2029810277 @default.
• W1990014307 hasRelatedWork W2031382568 @default.
• W1990014307 hasRelatedWork W2078964515 @default.
• W1990014307 hasRelatedWork W2104296830 @default.
• W1990014307 hasRelatedWork W2122069281 @default.
• W1990014307 hasRelatedWork W2167627570 @default.
• W1990014307 hasRelatedWork W2325531676 @default.
• W1990014307 hasRelatedWork W2396761760 @default.
• W1990014307 hasRelatedWork W2416042500 @default.
• W1990014307 hasRelatedWork W2552532596 @default.
• W1990014307 hasRelatedWork W2802629237 @default.
• W1990014307 hasRelatedWork W2889188325 @default.
• W1990014307 hasRelatedWork W2941471657 @default.
• W1990014307 hasRelatedWork W3007079619 @default.
• W1990014307 hasRelatedWork W3120449704 @default.

• next