Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990097457> ?p ?o ?g. }
- W1990097457 endingPage "296" @default.
- W1990097457 startingPage "292" @default.
- W1990097457 abstract "Despite the wide applicability and specificity of lectins to carbohydrate moieties, there are few lectin specific biosensors. This is attributed to the difficulty in defining the relevant experimental parameters to measure for sensing. We hereby describe the development of direct and indirect electrochemical sensors to determine the exact trace amounts of probarley lectin (ProBL) and its conversion product wheat germ agglutinin (WGA). In addition to WGA, the antigens (ProBL) employed in this study were over expressed in bacteria, isolated from protein bodies, and purified using immobilized N-acetylglusamine in order to obtain correctly folded active lectins. The amperometric immunosensor uses cell lines producing monoclonal antibody (mAB) to the pro-region of ProBL over expressed from Escherichia coli. The efficacy and sensing characteristics of the lectin were optimized using monoclonal antibody to WGA and the resulting sensor was found to detect only ProBL in the linear range 10−3–102 μg mL−1 and a detection limit of 10−3 μg mL−1." @default.
- W1990097457 created "2016-06-24" @default.
- W1990097457 creator A5034419498 @default.
- W1990097457 creator A5088399936 @default.
- W1990097457 date "2007-04-01" @default.
- W1990097457 modified "2023-09-27" @default.
- W1990097457 title "Electrochemical biosensors for monitoring the recognition of glycoprotein–lectin interactions" @default.
- W1990097457 cites W1923479745 @default.
- W1990097457 cites W1974385883 @default.
- W1990097457 cites W1974979380 @default.
- W1990097457 cites W1976819505 @default.
- W1990097457 cites W1993992450 @default.
- W1990097457 cites W2002639835 @default.
- W1990097457 cites W2004704094 @default.
- W1990097457 cites W2018319267 @default.
- W1990097457 cites W2024501283 @default.
- W1990097457 cites W2047881872 @default.
- W1990097457 cites W2051821293 @default.
- W1990097457 cites W2059718493 @default.
- W1990097457 cites W2061730295 @default.
- W1990097457 cites W2061831461 @default.
- W1990097457 cites W2070694674 @default.
- W1990097457 cites W2085103744 @default.
- W1990097457 cites W2086378969 @default.
- W1990097457 cites W2086552159 @default.
- W1990097457 cites W2089438534 @default.
- W1990097457 cites W2119742312 @default.
- W1990097457 cites W2136022619 @default.
- W1990097457 cites W2141351318 @default.
- W1990097457 doi "https://doi.org/10.1016/j.aca.2007.02.046" @default.
- W1990097457 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17386823" @default.
- W1990097457 hasPublicationYear "2007" @default.
- W1990097457 type Work @default.
- W1990097457 sameAs 1990097457 @default.
- W1990097457 citedByCount "24" @default.
- W1990097457 countsByYear W19900974572012 @default.
- W1990097457 countsByYear W19900974572013 @default.
- W1990097457 countsByYear W19900974572014 @default.
- W1990097457 countsByYear W19900974572017 @default.
- W1990097457 countsByYear W19900974572018 @default.
- W1990097457 countsByYear W19900974572019 @default.
- W1990097457 countsByYear W19900974572020 @default.
- W1990097457 countsByYear W19900974572021 @default.
- W1990097457 crossrefType "journal-article" @default.
- W1990097457 hasAuthorship W1990097457A5034419498 @default.
- W1990097457 hasAuthorship W1990097457A5088399936 @default.
- W1990097457 hasConcept C104317684 @default.
- W1990097457 hasConcept C106257327 @default.
- W1990097457 hasConcept C108625454 @default.
- W1990097457 hasConcept C119128265 @default.
- W1990097457 hasConcept C147483822 @default.
- W1990097457 hasConcept C153911025 @default.
- W1990097457 hasConcept C159654299 @default.
- W1990097457 hasConcept C160756335 @default.
- W1990097457 hasConcept C185592680 @default.
- W1990097457 hasConcept C203014093 @default.
- W1990097457 hasConcept C207545495 @default.
- W1990097457 hasConcept C2780175098 @default.
- W1990097457 hasConcept C2780758401 @default.
- W1990097457 hasConcept C43617362 @default.
- W1990097457 hasConcept C542903549 @default.
- W1990097457 hasConcept C54355233 @default.
- W1990097457 hasConcept C547475151 @default.
- W1990097457 hasConcept C55493867 @default.
- W1990097457 hasConcept C86803240 @default.
- W1990097457 hasConceptScore W1990097457C104317684 @default.
- W1990097457 hasConceptScore W1990097457C106257327 @default.
- W1990097457 hasConceptScore W1990097457C108625454 @default.
- W1990097457 hasConceptScore W1990097457C119128265 @default.
- W1990097457 hasConceptScore W1990097457C147483822 @default.
- W1990097457 hasConceptScore W1990097457C153911025 @default.
- W1990097457 hasConceptScore W1990097457C159654299 @default.
- W1990097457 hasConceptScore W1990097457C160756335 @default.
- W1990097457 hasConceptScore W1990097457C185592680 @default.
- W1990097457 hasConceptScore W1990097457C203014093 @default.
- W1990097457 hasConceptScore W1990097457C207545495 @default.
- W1990097457 hasConceptScore W1990097457C2780175098 @default.
- W1990097457 hasConceptScore W1990097457C2780758401 @default.
- W1990097457 hasConceptScore W1990097457C43617362 @default.
- W1990097457 hasConceptScore W1990097457C542903549 @default.
- W1990097457 hasConceptScore W1990097457C54355233 @default.
- W1990097457 hasConceptScore W1990097457C547475151 @default.
- W1990097457 hasConceptScore W1990097457C55493867 @default.
- W1990097457 hasConceptScore W1990097457C86803240 @default.
- W1990097457 hasIssue "2" @default.
- W1990097457 hasLocation W19900974571 @default.
- W1990097457 hasLocation W19900974572 @default.
- W1990097457 hasOpenAccess W1990097457 @default.
- W1990097457 hasPrimaryLocation W19900974571 @default.
- W1990097457 hasRelatedWork W1963499284 @default.
- W1990097457 hasRelatedWork W2006623899 @default.
- W1990097457 hasRelatedWork W2049549607 @default.
- W1990097457 hasRelatedWork W2051749473 @default.
- W1990097457 hasRelatedWork W2063777900 @default.
- W1990097457 hasRelatedWork W2072358059 @default.
- W1990097457 hasRelatedWork W2109511736 @default.
- W1990097457 hasRelatedWork W2334396649 @default.
- W1990097457 hasRelatedWork W2413403991 @default.