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- W1990097466 abstract "A novel procedure for the authentication of frigate tunas (Auxis thazard and Auxis rochei) in commercially canned products has been developed. Three mitochondrial regions were simultaneously amplified by multiplex-Polymerase Chain Reaction, one corresponding to the small rRNA 12S subunit as a positive amplification control and two species-specific fragments corresponding to cytochrome b for A. rochei and ATPase 6 for A. thazard, respectively. Testing of two different detection systems revealed the fluorescence-based approach as the most sensitive. The results demonstrate that this rapid, low-cost methodology is a reliable molecular tool for direct application in the authentication of canned products. Keywords: Auxis thazard; Auxis rochei; cytochrome b; ATPase 6; multiplex-PCR; canned products" @default.
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- W1990097466 date "2004-11-10" @default.
- W1990097466 modified "2023-10-16" @default.
- W1990097466 title "Novel Method for the Authentication of Frigate Tunas (<i>Auxis thazard</i> and <i>Auxis rochei</i>) in Commercial Canned Products" @default.
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- W1990097466 doi "https://doi.org/10.1021/jf0492868" @default.
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