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- W1990135473 abstract "The membrane-attached polysomes of the liver cells are assumed to be the intracellular site of albumin synthesis (for recent critical review see Campbell (1)) . The histological localization of albumin by fluorescent antisera has been described repeatedly (3, 5-7) . It is not possible, however, to decide whether albumin localized in the cell by this technique is newly synthesized, or taken up by the cells from the blood, or simply adsorbed during preparation . In radioautographs we observed predominant labeling of the periportal area of the liver lobules after intraportal injection of leucine-3H, whereas intracaval injection led to uniform labeling throughout the lobule . The way of administration of the tracer thus permits a study of protein synthesis of selected regions of the liver lobule . Recently, the isolation of radiochemically pure albumin from liver homogenates and a method to determine the proportion of albumin to total protein synthesis have been described (9) . In this report the ratio of albumin to total protein synthesis after intraportal injection was compared to that after intracaval injection . By combining these results with the radioautographic observations, it was possible to decide whether or not albumin synthesis was distributed uniformly throughout the liver lobule ." @default.
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- W1990135473 date "1970-10-01" @default.
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- W1990135473 title "THE DISTRIBUTION OF ALBUMIN SYNTHESIS THROUGHOUT THE LIVER LOBULE" @default.
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- W1990135473 doi "https://doi.org/10.1083/jcb.47.1.285" @default.
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