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• W1990149904 abstract "Abstract In this study, we address the hypothesis that it is possible to exploit genetic/functional variation in parental Chinese hamster ovary (CHO) cell populations to isolate clonal derivatives that exhibit superior, heritable attributes for biomanufacturing—new parental cell lines which are inherently more “fit for purpose.” One‐hundred and ninety‐nine CHOK1SV clones were isolated from a donor CHOK1SV parental population by limiting dilution cloning and microplate image analysis, followed by primary analysis of variation in cell‐specific proliferation rate during extended deep‐well microplate suspension culture of individual clones to accelerate genetic drift in isolated cultures. A subset of 100 clones were comparatively evaluated for transient production of a recombinant monoclonal antibody (Mab) and green fluorescent protein following transfection of a plasmid vector encoding both genes. The heritability of both cell‐specific proliferation rate and Mab production was further assessed using a subset of 23 clones varying in functional capability that were subjected to cell culture regimes involving both cryopreservation and extended sub‐culture. These data showed that whilst differences in transient Mab production capability were not heritable per se, clones exhibiting heritable variation in specific proliferation rate, endocytotic transfectability and N ‐glycan processing were identified. Finally, for clonal populations most “evolved” by extended sub‐culture in vitro we investigated the relationship between cellular protein biomass content, specific proliferation rate and cell surface N‐glycosylation. Rapid‐specific proliferation rate was inversely correlated to CHO cell size and protein content, and positively correlated to cell surface glycan content, although substantial clone‐specific variation in ability to accumulate cell biomass was evident. Taken together, our data reveal the dynamic nature of the CHO cell functional genome and the potential to evolve and isolate CHO cell variants with improved functional properties in vitro. Biotechnol. Bioeng. 2013; 110: 260–274. © 2012 Wiley Periodicals, Inc." @default.
• W1990149904 created "2016-06-24" @default.
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• W1990149904 date "2012-08-16" @default.
• W1990149904 modified "2023-10-17" @default.
• W1990149904 title "Functional heterogeneity and heritability in CHO cell populations" @default.
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• W1990149904 cites W1967156547 @default.
• W1990149904 cites W1973764237 @default.
• W1990149904 cites W1979371976 @default.
• W1990149904 cites W1980167726 @default.
• W1990149904 cites W1987221915 @default.
• W1990149904 cites W1987775447 @default.
• W1990149904 cites W1989752449 @default.
• W1990149904 cites W1996759600 @default.
• W1990149904 cites W1998051089 @default.
• W1990149904 cites W1999696025 @default.
• W1990149904 cites W1999942506 @default.
• W1990149904 cites W2012416465 @default.
• W1990149904 cites W2013324041 @default.
• W1990149904 cites W2014496483 @default.
• W1990149904 cites W2033032210 @default.
• W1990149904 cites W2034979835 @default.
• W1990149904 cites W2036934214 @default.
• W1990149904 cites W2047511535 @default.
• W1990149904 cites W2048941695 @default.
• W1990149904 cites W2049728326 @default.
• W1990149904 cites W2050826226 @default.
• W1990149904 cites W2056257023 @default.
• W1990149904 cites W2057848231 @default.
• W1990149904 cites W2060985723 @default.
• W1990149904 cites W2066499437 @default.
• W1990149904 cites W2075251342 @default.
• W1990149904 cites W2075414171 @default.
• W1990149904 cites W2079023951 @default.
• W1990149904 cites W2092333126 @default.
• W1990149904 cites W2093343938 @default.
• W1990149904 cites W2094535938 @default.
• W1990149904 cites W2097259163 @default.
• W1990149904 cites W2100380298 @default.
• W1990149904 cites W2104009579 @default.
• W1990149904 cites W2104929243 @default.
• W1990149904 cites W2105081087 @default.
• W1990149904 cites W2107972234 @default.
• W1990149904 cites W2109208152 @default.
• W1990149904 cites W2109716542 @default.
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• W1990149904 cites W2114499303 @default.
• W1990149904 cites W2114632170 @default.
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• W1990149904 cites W2132496668 @default.
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• W1990149904 doi "https://doi.org/10.1002/bit.24621" @default.
• W1990149904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22833427" @default.
• W1990149904 hasPublicationYear "2012" @default.
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