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- W1990205100 abstract "The described method allowed good control of the degree of iodination which can be calculated fairly accurately if it is assumed that incorporation of carrier iodine parallels that of radioactive iodine. The possibility of denaturation or other damage to the protein is diminished by the use of an iodination solution free of oxidizing agents other than iodine itself. Using a crystalline glucagon preparation, incorporation of 15 to 25% of the totally employed radioactivity was obtained. La méthode décrite permet une bonne détermination du degré d'oiduration qu'on peut calculer avec une bonne précision si on admet que l'incorporation de l'iode entraîneur est parallèle à celle de l'iode radioactif. Le risque de dénaturation ou autre détérioration de la protéine est réduit par l'utilisation d'une solution d'ioduration dépourvue d'agent oxydant autre que l'iode lui-même. Avec une préparation de glucagon cristallin, on obtient l'incorporation de 15 à 25% du corps radioactif employé. OпиcaH;H;ый M;eT;oд пoзB;oляeT; кoH;T;poлиpoB;aT;ь cT;eпeH;ь иoдиpoB;aH;ия, кoT;opaя M;oжeT; быT;ь packhcy;иT;aH;a дoB;oльH;o T;okhcy;H;o, ecли пpиH;яT;ь, khcy;T;o пoглoщeH;иe иoдa H;ocиT;eля пpcиcчoдиT; пapaллeльH;o пoглoщeH;ию paдиoaкT;иB;H;oгo иoдa. Для yM;eH;ьшeH;ия B;oзM;oжH;ocT;и дeH;aT;ypиpoB;aH;ия или дpyгoгo пoB;peждeH;ия бeлкa пpиM;eH;яюT; pacT;B;op, cB;oбoдH;ый oT; B;cякич дpyгич oкиcляющич aгeH;T;oB;, кpoM;e иoдa. ПyT;eM; пpиM;eH;eH;ия пpeпapaT;a кpиcT;aллиkhcy;ecкoгo глюкaгoH;a yдaлocь пoлykhcy;иT;ь пoглoщeH;иe oT; 15 дo 25% B;ceй B;B;oдиM;oй paдиoaкT;иB;H;ocT;и. Die beschriebene Methode gestattet eine gute Kontrolle des Jodierungsgrades, welcher sich ziemlich genau berechnen lässt, wenn angenommen wird, dass der Einbau des inaktiven Jods mit dem des radioaktiven Jods parallel verläuft. Die Möglichkeit einer Denaturierung oder einer anderen Schädigung des Proteins wird herabgesetzt, wenn die Jodierungslösung ausser dem Jod kein weiteres oxydierendes Agens enthält. Bei Verwendung von kristallinen Glucagon-Präparaten gelang der Einbau von 15–25% des insgesamt verwendeten radioaktiven Materials." @default.
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- W1990205100 date "1957-04-01" @default.
- W1990205100 modified "2023-09-27" @default.
- W1990205100 title "An improved technique for labelling proteins with I131" @default.
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- W1990205100 doi "https://doi.org/10.1016/0020-708x(57)90027-3" @default.
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