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- W1990214181 abstract "The cornea is the most frequently transplanted solid organ tissue. Despite the presence of blood group ABO antigens in the corneal epithelium and endothelium, blood group matching is not usually considered necessary in corneal transplantation. Clinical studies have yielded different results, some showing a favourable effect of ABO matching on graft survival and others reporting no benefit. We examined the effect of ABO mismatching on cultured human corneal epithelial cells. 15 primary human corneal epithelial cultures, confirmed by cytokeratin staining, were established from 15 donor rims remaining after corneal transplantation. At confluence (3 weeks), the cells were harvested and suspended in buffered saline (1 10 cells per mL). Cells from each culture were stained for blood group A, B, and H(O) antigens with immunoperoxidase, and tested for agglutination with anti A, B, and H(O) antibodies (Zymed Laboratories, San Francisco, CA, USA) by mixing 100 L of cell suspension (figure A) with 100 L of antibody on a glass slide. Trypanblue dye exclusion was used to assess cell viability. Of the 15 cell cultures, three were agglutinated with antiA, ten with anti-H, and two with anti-B. None of the cell cultures agglutinated with both anti-A and anti-B. After this study, donor blood groups were tracked from eye-bank records and were found to correspond with the blood groups determined by agglutination of corneal epithelial cells. Trypan-blue dye exclusion showed a high proportion of dead cells in the agglutinated clumps of epithelial cells (figure B). Determination of ABO groups by agglutination and immunoperoxidase staining also corresponded in all samples. This study shows a definite harmful effect of blood group ABO mismatching on corneal epithelial cells. The plasmoid aqueous that fills the anterior chamber immediately after surgery is known to contain a high concentration of immunoglobulins. It is therefore possible for ABO mismatching to adversely affect corneal transplants, particularly in high-risk situations with vascularised recipient beds. Corneal endothelial cells also show blood group antigens but are difficult to grow in culture; whether corneal endothelial cells would be similarly affected is not known but it is known that onset of corneal epithelial rejection can trigger endothelial rejection." @default.
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- W1990214181 date "1998-11-01" @default.
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- W1990214181 title "Adverse effect of blood group ABO mismatching on corneal epithelial cells" @default.
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- W1990214181 doi "https://doi.org/10.1016/s0140-6736(05)61448-1" @default.
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