FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990215212> ?p ?o ?g. }

• W1990215212 endingPage "165" @default.
• W1990215212 startingPage "159" @default.
• W1990215212 abstract "Smooth muscle cell (SMC) hyperplasia is an important component of vascular remodeling in chronic hypoxic pulmonary hypertension. The mechanisms underlying SMC proliferation in the remodeling process are poorly understood, but may involve insulin-like growth factor I (IGF-I). This study investigates the potential proliferative effects of IGF-I on SMC cultured from the pulmonary arteries (PA) of neonatal calves. We hypothesized that IGF-I stimulates PA SMC proliferation through a protein kinase C (PKC)-indepenent pathway, but that PKC activation would augment this proliferative response. Incorporation of 3H-thymidine was used as an index of cellular prohteration, and was correlated with subsequent changes in cell counts. Under serum-free conditions, IGF-I (100 ng/ml) induced a 6-fold increase in thymidine incorporation by quiescent PA SMC. This stimulation was not blocked by dihydrosphingosine, an inhibitor of PKC activation. Phorbol myristate acetate (PMA) (1 nM), a membrane-permeable PKC activator, induced a 12-fold increase in thymidine incorporation which was 70% inhibited by dihydrosphingosine. Co-incubation with IGF-I and PMA caused a 60-fold increase in thymidine incorporation, which was 30% inhibited by dihydrosphingosine. This synergistic increase in thymidine incorporation was associated with a subsequent significant increase in cell number. PKC-downregulated cells (1,000 nM PMA × 30 hr) proliferated in response to IGF-I but not PMA, and did not demonstrate synergism with the combination of IGF-I and PMA. The threshold concentrations of IGF-I and PMA for synergism were approximately 1 ng/ml and 1 pM, respectively. We conclude that IGF-I stimulates neonatal PA SMC proliferation via a PKC-independent pathway, and that trace amounts of PKC activators are capable of synergistically augmenting this response. We speculate that the synergistic stimulation of SMC proliferation by IGF-I and PKC activators may play an important role in hypertensive pulmonary vascular remodeling." @default.
• W1990215212 created "2016-06-24" @default.
• W1990215212 creator A5011168502 @default.
• W1990215212 creator A5014076592 @default.
• W1990215212 creator A5048362132 @default.
• W1990215212 creator A5056518661 @default.
• W1990215212 creator A5059343570 @default.
• W1990215212 creator A5084022307 @default.
• W1990215212 date "1990-07-01" @default.
• W1990215212 modified "2023-10-15" @default.
• W1990215212 title "Insulin-Like growth factor I and protein kinase C activation stimulate pulmonary artery smooth muscle cell proliferation through separate but synergistic pathways" @default.
• W1990215212 cites W1492241928 @default.
• W1990215212 cites W1602448176 @default.
• W1990215212 cites W1964839077 @default.
• W1990215212 cites W1965368707 @default.
• W1990215212 cites W1968113908 @default.
• W1990215212 cites W1975797622 @default.
• W1990215212 cites W1978036864 @default.
• W1990215212 cites W1983312218 @default.
• W1990215212 cites W2001266486 @default.
• W1990215212 cites W2009373735 @default.
• W1990215212 cites W2019617436 @default.
• W1990215212 cites W2019911238 @default.
• W1990215212 cites W2024528924 @default.
• W1990215212 cites W2040052703 @default.
• W1990215212 cites W2041402293 @default.
• W1990215212 cites W2043037423 @default.
• W1990215212 cites W2049680420 @default.
• W1990215212 cites W2051200506 @default.
• W1990215212 cites W2061574494 @default.
• W1990215212 cites W2061914961 @default.
• W1990215212 cites W2062637379 @default.
• W1990215212 cites W2066445704 @default.
• W1990215212 cites W2068381364 @default.
• W1990215212 cites W2072159062 @default.
• W1990215212 cites W207524507 @default.
• W1990215212 cites W2095302611 @default.
• W1990215212 cites W2142017330 @default.
• W1990215212 cites W2174542542 @default.
• W1990215212 cites W2264216940 @default.
• W1990215212 cites W2334897036 @default.
• W1990215212 cites W2416865393 @default.
• W1990215212 cites W2465806616 @default.
• W1990215212 doi "https://doi.org/10.1002/jcp.1041440121" @default.
• W1990215212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2365741" @default.
• W1990215212 hasPublicationYear "1990" @default.
• W1990215212 type Work @default.
• W1990215212 sameAs 1990215212 @default.
• W1990215212 citedByCount "49" @default.
• W1990215212 countsByYear W19902152122012 @default.
• W1990215212 countsByYear W19902152122015 @default.
• W1990215212 countsByYear W19902152122016 @default.
• W1990215212 crossrefType "journal-article" @default.
• W1990215212 hasAuthorship W1990215212A5011168502 @default.
• W1990215212 hasAuthorship W1990215212A5014076592 @default.
• W1990215212 hasAuthorship W1990215212A5048362132 @default.
• W1990215212 hasAuthorship W1990215212A5056518661 @default.
• W1990215212 hasAuthorship W1990215212A5059343570 @default.
• W1990215212 hasAuthorship W1990215212A5084022307 @default.
• W1990215212 hasConcept C126322002 @default.
• W1990215212 hasConcept C134018914 @default.
• W1990215212 hasConcept C170493617 @default.
• W1990215212 hasConcept C185592680 @default.
• W1990215212 hasConcept C195794163 @default.
• W1990215212 hasConcept C202751555 @default.
• W1990215212 hasConcept C24998067 @default.
• W1990215212 hasConcept C2775960820 @default.
• W1990215212 hasConcept C2779395532 @default.
• W1990215212 hasConcept C2780043322 @default.
• W1990215212 hasConcept C2780689927 @default.
• W1990215212 hasConcept C2992686903 @default.
• W1990215212 hasConcept C55493867 @default.
• W1990215212 hasConcept C62112901 @default.
• W1990215212 hasConcept C62478195 @default.
• W1990215212 hasConcept C64241487 @default.
• W1990215212 hasConcept C71924100 @default.
• W1990215212 hasConcept C86803240 @default.
• W1990215212 hasConcept C88045685 @default.
• W1990215212 hasConcept C95444343 @default.
• W1990215212 hasConceptScore W1990215212C126322002 @default.
• W1990215212 hasConceptScore W1990215212C134018914 @default.
• W1990215212 hasConceptScore W1990215212C170493617 @default.
• W1990215212 hasConceptScore W1990215212C185592680 @default.
• W1990215212 hasConceptScore W1990215212C195794163 @default.
• W1990215212 hasConceptScore W1990215212C202751555 @default.
• W1990215212 hasConceptScore W1990215212C24998067 @default.
• W1990215212 hasConceptScore W1990215212C2775960820 @default.
• W1990215212 hasConceptScore W1990215212C2779395532 @default.
• W1990215212 hasConceptScore W1990215212C2780043322 @default.
• W1990215212 hasConceptScore W1990215212C2780689927 @default.
• W1990215212 hasConceptScore W1990215212C2992686903 @default.
• W1990215212 hasConceptScore W1990215212C55493867 @default.
• W1990215212 hasConceptScore W1990215212C62112901 @default.
• W1990215212 hasConceptScore W1990215212C62478195 @default.
• W1990215212 hasConceptScore W1990215212C64241487 @default.
• W1990215212 hasConceptScore W1990215212C71924100 @default.
• W1990215212 hasConceptScore W1990215212C86803240 @default.
• W1990215212 hasConceptScore W1990215212C88045685 @default.

• next