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- W1990220836 abstract "Fluorescence and photochemical properties of phytochromes A and B (phyA and phyB) have been investigated in etiolated pea (Pisum sativum L.) seedlings by comparing wild-type (wt) seedlings with mutants lacking phyB (lv-5) or phyA (fun1-1), and with the double mutant. The red-light (R)-absorbing form of phytochrome (Pr) is characterized by four major parameters: position of the emission maximum at 85 K (λmax), total phytochrome content ([Ptot]); extent of the Pr photoransformation into the first product, lumi-R, at 85 K (γ1), and into the far-red light (FR)-absorbing form (Pfr) at 273 K (γ2). Phytochrome cannot be detected in the lv-5 fun1-1 double mutant, allowing specific characterization of phyA and phyB in the single mutants. Thus, for the first time we are able to compare in planta the properties of native phyA and phyB. Our results show the phyA population to be heterogenous both in distribution and properties. [PhyAtot] is highest in the upper stem (5.23 rel. units), with λmax of 687 nm, γ1=0.43 and γ2=0.72, and 3.5-fold lower in the lower stem and roots, with γmax blue-shifted by more than 1 nm, γ1=0.3 and γ2=0.7. In contrast, phyB is present at a low level throughout the plant (0.3–0.4 rel. units), with γmax of 683 nm, γ1 of 0.05 and γ2 of 0.3–0.35. It is also characterized by an earlier red drop in the dependence of γ2 on the wavelength of the actinic light compared to phyA. Experiments with light-induced phytochrome destruction confirm previous findings that the majority of phyA, unlike phyB, is light-labile. The variation in properties of phyA is interpreted in terms of two species, phyA′ and phyA″. PhyA′ is relatively abundant, is distributed mainly in the upper stem, is light-labile and has a high γ1 value (≈0.5), whereas phyA″ is less abundant, is distributed more or less evenly throughout the plant, is relatively light-stable and has a low γ1 value (<0.05). It is suggested that the lower photochemical activity of phyB at ambient temperatures (low γ2) and its earlier red drop (which is likely to be due to the blue-shift in the absorption spectra of phyB Pr and Pfr) might explain, at least partially, the lower effectiveness of phyB under R and its lack of effect under FR." @default.
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- W1990220836 date "1999-04-01" @default.
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- W1990220836 title "Fluorescence and photochemical properties of phytochromes A and B in etiolated pea seedlings" @default.
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- W1990220836 doi "https://doi.org/10.1016/s1011-1344(99)00061-5" @default.
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