Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990230495> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W1990230495 endingPage "106" @default.
- W1990230495 startingPage "93" @default.
- W1990230495 abstract "The immunoassay method in which 125I-labeled staphylococcal Protein A ([125I]PA) serves as a general tracer has been extended to include goat and sheep IgG antibodies. Goat and sheep IgG normally do not react significantly with PA. However, once IgG antibody is bound to immobilized antigen or hapten, binding of [125I]PA is enhanced markedly. Binding efficiencies of [125I]PA to immune complexed goat anti-human IgM, human IgE, methotrexate and sheep anti-IgE were determined and compared quantitatively to rabbit IgG with the corresponding specificity. Immunoassays were developed based on the inhibition of [125I]PA binding as a measure of antibody inhibition by fluid-phase homologous ligand. It terms of sensitivity and specificity, assays using goat and sheep antibodies were comparable to assays developed using rabbit IgG. Goat antibody to the monovalent hapten methotrexate behaved anomalously: for each concentration of IgG tested, there was an optimal amount of methotrexate beads that gave maximum binding of [125I]PA. In the other immune systems, for each antibody concentration maximum binding of tracer was a function only of the amount of immobilized antigen added. In contrast to the results obtained with solid-phase antigen, solutions containing antibody and amounts of antigen ranging from large antigen excess to antibody excess failed to react significantly with PA or [125I]PA." @default.
- W1990230495 created "2016-06-24" @default.
- W1990230495 creator A5064669502 @default.
- W1990230495 date "1980-05-01" @default.
- W1990230495 modified "2023-09-24" @default.
- W1990230495 title "125I-labeled protein A as a general tracer in immunoassay: Suitability of goat and sheep antibodies" @default.
- W1990230495 cites W1966349808 @default.
- W1990230495 cites W1981881341 @default.
- W1990230495 cites W1991929713 @default.
- W1990230495 cites W2005833822 @default.
- W1990230495 cites W2016133866 @default.
- W1990230495 cites W2036517856 @default.
- W1990230495 cites W2044708909 @default.
- W1990230495 cites W2084233148 @default.
- W1990230495 cites W2086068049 @default.
- W1990230495 doi "https://doi.org/10.1016/0022-1759(80)90163-5" @default.
- W1990230495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6155411" @default.
- W1990230495 hasPublicationYear "1980" @default.
- W1990230495 type Work @default.
- W1990230495 sameAs 1990230495 @default.
- W1990230495 citedByCount "34" @default.
- W1990230495 countsByYear W19902304952014 @default.
- W1990230495 countsByYear W19902304952016 @default.
- W1990230495 crossrefType "journal-article" @default.
- W1990230495 hasAuthorship W1990230495A5064669502 @default.
- W1990230495 hasConcept C141105273 @default.
- W1990230495 hasConcept C147483822 @default.
- W1990230495 hasConcept C153911025 @default.
- W1990230495 hasConcept C159654299 @default.
- W1990230495 hasConcept C185592680 @default.
- W1990230495 hasConcept C203014093 @default.
- W1990230495 hasConcept C2780420688 @default.
- W1990230495 hasConcept C2780898057 @default.
- W1990230495 hasConcept C29144244 @default.
- W1990230495 hasConcept C43617362 @default.
- W1990230495 hasConcept C55493867 @default.
- W1990230495 hasConcept C86803240 @default.
- W1990230495 hasConcept C8891405 @default.
- W1990230495 hasConceptScore W1990230495C141105273 @default.
- W1990230495 hasConceptScore W1990230495C147483822 @default.
- W1990230495 hasConceptScore W1990230495C153911025 @default.
- W1990230495 hasConceptScore W1990230495C159654299 @default.
- W1990230495 hasConceptScore W1990230495C185592680 @default.
- W1990230495 hasConceptScore W1990230495C203014093 @default.
- W1990230495 hasConceptScore W1990230495C2780420688 @default.
- W1990230495 hasConceptScore W1990230495C2780898057 @default.
- W1990230495 hasConceptScore W1990230495C29144244 @default.
- W1990230495 hasConceptScore W1990230495C43617362 @default.
- W1990230495 hasConceptScore W1990230495C55493867 @default.
- W1990230495 hasConceptScore W1990230495C86803240 @default.
- W1990230495 hasConceptScore W1990230495C8891405 @default.
- W1990230495 hasIssue "2" @default.
- W1990230495 hasLocation W19902304951 @default.
- W1990230495 hasLocation W19902304952 @default.
- W1990230495 hasOpenAccess W1990230495 @default.
- W1990230495 hasPrimaryLocation W19902304951 @default.
- W1990230495 hasRelatedWork W1501159819 @default.
- W1990230495 hasRelatedWork W1511442365 @default.
- W1990230495 hasRelatedWork W1630499719 @default.
- W1990230495 hasRelatedWork W1980647413 @default.
- W1990230495 hasRelatedWork W1984050845 @default.
- W1990230495 hasRelatedWork W1990230495 @default.
- W1990230495 hasRelatedWork W1999009491 @default.
- W1990230495 hasRelatedWork W2030805283 @default.
- W1990230495 hasRelatedWork W2107563424 @default.
- W1990230495 hasRelatedWork W2394742267 @default.
- W1990230495 hasVolume "34" @default.
- W1990230495 isParatext "false" @default.
- W1990230495 isRetracted "false" @default.
- W1990230495 magId "1990230495" @default.
- W1990230495 workType "article" @default.