FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990236425> ?p ?o ?g. }

• W1990236425 endingPage "105" @default.
• W1990236425 startingPage "99" @default.
• W1990236425 abstract "M-IL-2((88)Arg, (125)Ala) is a fusion protein comprising melittin genetically linked to a mutant human interleukin 2((88)Arg, (125)Ala). In this study, we constructed an expression system of M-IL-2((88)Arg, (125)Ala) in Pichia pastoris: GS115/pPICZα A/M-IL-2((88)Arg, (125)Ala), and achieved the high-level expression of the fusion protein. The maximum yield of the fusion protein M-IL-2((88)Arg, (125)Ala) reached up to 814.5mg/L, higher than the system in Escherichiacoli. The fusion protein was purified by means of ammonium sulfate fractionation, dialysis and nickel ion affinity chromatography. The molecular weight of the fusion protein is about 26kDa, conforming the theoretical value. And M-IL-2((88)Arg, (125)Ala) possesses strong antigen-specificity by Western blot detection. Bioassay results indicated that the fusion protein could directly inhibit the growth of human ovarian cancer SKOV3 cells and Hela cells in vitro. This study provides an alternative strategy for large-scale production of bioactive M-IL-2((88)Arg, (125)Ala) using P. pastoris as an expression host and paves the way to clinical practice." @default.
• W1990236425 created "2016-06-24" @default.
• W1990236425 creator A5024268493 @default.
• W1990236425 creator A5025665661 @default.
• W1990236425 creator A5029348144 @default.
• W1990236425 creator A5054419225 @default.
• W1990236425 creator A5062674156 @default.
• W1990236425 creator A5070887372 @default.
• W1990236425 creator A5081784254 @default.
• W1990236425 creator A5084000653 @default.
• W1990236425 date "2014-09-01" @default.
• W1990236425 modified "2023-09-24" @default.
• W1990236425 title "High-level expression, purification and study of bioactivity of fusion protein M-IL-2(88Arg, 125Ala) in Pichia pastoris" @default.
• W1990236425 cites W1508418323 @default.
• W1990236425 cites W1543267956 @default.
• W1990236425 cites W1966999658 @default.
• W1990236425 cites W1975568557 @default.
• W1990236425 cites W1979343444 @default.
• W1990236425 cites W1980164108 @default.
• W1990236425 cites W1982139065 @default.
• W1990236425 cites W1985981664 @default.
• W1990236425 cites W1989727905 @default.
• W1990236425 cites W1992707998 @default.
• W1990236425 cites W1993051112 @default.
• W1990236425 cites W2013790143 @default.
• W1990236425 cites W2016869568 @default.
• W1990236425 cites W2037034824 @default.
• W1990236425 cites W2045046170 @default.
• W1990236425 cites W2051636382 @default.
• W1990236425 cites W2051962533 @default.
• W1990236425 cites W2061813158 @default.
• W1990236425 cites W2072309742 @default.
• W1990236425 cites W2073268080 @default.
• W1990236425 cites W2076123100 @default.
• W1990236425 cites W2081635117 @default.
• W1990236425 cites W2083905477 @default.
• W1990236425 cites W2120357935 @default.
• W1990236425 cites W2160873944 @default.
• W1990236425 cites W2170577113 @default.
• W1990236425 cites W2222964685 @default.
• W1990236425 doi "https://doi.org/10.1016/j.pep.2014.06.008" @default.
• W1990236425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24955549" @default.
• W1990236425 hasPublicationYear "2014" @default.
• W1990236425 type Work @default.
• W1990236425 sameAs 1990236425 @default.
• W1990236425 citedByCount "8" @default.
• W1990236425 countsByYear W19902364252015 @default.
• W1990236425 countsByYear W19902364252017 @default.
• W1990236425 countsByYear W19902364252018 @default.
• W1990236425 countsByYear W19902364252021 @default.
• W1990236425 countsByYear W19902364252022 @default.
• W1990236425 countsByYear W19902364252023 @default.
• W1990236425 crossrefType "journal-article" @default.
• W1990236425 hasAuthorship W1990236425A5024268493 @default.
• W1990236425 hasAuthorship W1990236425A5025665661 @default.
• W1990236425 hasAuthorship W1990236425A5029348144 @default.
• W1990236425 hasAuthorship W1990236425A5054419225 @default.
• W1990236425 hasAuthorship W1990236425A5062674156 @default.
• W1990236425 hasAuthorship W1990236425A5070887372 @default.
• W1990236425 hasAuthorship W1990236425A5081784254 @default.
• W1990236425 hasAuthorship W1990236425A5084000653 @default.
• W1990236425 hasConcept C104317684 @default.
• W1990236425 hasConcept C111829193 @default.
• W1990236425 hasConcept C123894998 @default.
• W1990236425 hasConcept C153911025 @default.
• W1990236425 hasConcept C179247698 @default.
• W1990236425 hasConcept C181199279 @default.
• W1990236425 hasConcept C185592680 @default.
• W1990236425 hasConcept C2776415932 @default.
• W1990236425 hasConcept C2781372317 @default.
• W1990236425 hasConcept C33161422 @default.
• W1990236425 hasConcept C40767141 @default.
• W1990236425 hasConcept C55493867 @default.
• W1990236425 hasConcept C86803240 @default.
• W1990236425 hasConceptScore W1990236425C104317684 @default.
• W1990236425 hasConceptScore W1990236425C111829193 @default.
• W1990236425 hasConceptScore W1990236425C123894998 @default.
• W1990236425 hasConceptScore W1990236425C153911025 @default.
• W1990236425 hasConceptScore W1990236425C179247698 @default.
• W1990236425 hasConceptScore W1990236425C181199279 @default.
• W1990236425 hasConceptScore W1990236425C185592680 @default.
• W1990236425 hasConceptScore W1990236425C2776415932 @default.
• W1990236425 hasConceptScore W1990236425C2781372317 @default.
• W1990236425 hasConceptScore W1990236425C33161422 @default.
• W1990236425 hasConceptScore W1990236425C40767141 @default.
• W1990236425 hasConceptScore W1990236425C55493867 @default.
• W1990236425 hasConceptScore W1990236425C86803240 @default.
• W1990236425 hasLocation W19902364251 @default.
• W1990236425 hasLocation W19902364252 @default.
• W1990236425 hasOpenAccess W1990236425 @default.
• W1990236425 hasPrimaryLocation W19902364251 @default.
• W1990236425 hasRelatedWork W2362101020 @default.
• W1990236425 hasRelatedWork W2362612214 @default.
• W1990236425 hasRelatedWork W2371993564 @default.
• W1990236425 hasRelatedWork W2373329635 @default.
• W1990236425 hasRelatedWork W2381845779 @default.
• W1990236425 hasRelatedWork W2381958483 @default.
• W1990236425 hasRelatedWork W2393451491 @default.

• next