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• W1990242543 endingPage "6012" @default.
• W1990242543 startingPage "6006" @default.
• W1990242543 abstract "Carboxylic group-functionalized single-walled carbon nanotubes (SWNTs) were assembled vertically on the glassy carbon electrode using ethylenediamine as linking agent to fabricate an aligned electrode (SWNTE). Single-stranded DNA (ssDNA) wrapped around the SWNTs to form ssDNA-wrapped SWNTE structures based on the interaction between ssDNA and SWNT. A sensitive differential pulse voltammetric (DPV) response was obtained at the ssDNA-wrapped SWNTE owing to the electrooxidation of guanine bases. Double-stranded DNA (dsDNA) was formed when ssDNA on the ssDNA-wrapped SWNTE was hybridized with complementary ssDNA (cDNA). The dsDNA was removed from the SWNTs by undergoing a process of preconditioning at -0.6 V. Consequentially, the DPV response of guanine bases decreased. The used SWNTE could be renewed easily via ultrasonically rinsing. On the basis of this mechanism, a label-free and readily reusable electrochemical DNA hybridization biosensor was designed by directly monitoring the current change of guanine bases. Under optimum conditions, the plot of the measurement signal of guanine bases versus the cDNA concentrations was a good straight line in the range of 40-110 nM with a detection limit of 20 nM (3s). The biosensor can be switched to detect different target DNAs easily." @default.
• W1990242543 created "2016-06-24" @default.
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• W1990242543 date "2009-06-29" @default.
• W1990242543 modified "2023-10-16" @default.
• W1990242543 title "Readily Reusable Electrochemical DNA Hybridization Biosensor Based on the Interaction of DNA with Single-Walled Carbon Nanotubes" @default.
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• W1990242543 doi "https://doi.org/10.1021/ac802026j" @default.
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• W1990242543 hasPublicationYear "2009" @default.
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