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• W1990268265 startingPage "236" @default.
• W1990268265 abstract "Intact HEK293 cells and B103 neuroblastoma cells possess high basal concentrations of the established second messengers cAMP and cGMP and of the emerging second messengers cCMP and cUMP. We asked the question which nucleotidyl cyclase accounts for the high basal cNMP concentrations. Activators and inhibitors of soluble guanylyl cyclase had no major effects on cNMPs, and the activator of membranous adenylyl cyclase forskolin increased only cAMP. Addition of bicarbonate to medium increased, whereas removal of bicarbonate decreased levels of all four cNMPs. The inhibitor of soluble adenylyl cyclase, 2-(1H-benzo[d]imidazol-2-ylthio)-N′-(5-bromo-2-hydroxybenzylidene) propanehydrazide (KH7), reduced bicarbonate-stimulated cNMPs. In conclusion, bicarbonate-stimulated soluble adenylyl cyclase plays an important role in the regulation of basal cellular cNMP levels, most notably cCMP and cUMP." @default.
• W1990268265 created "2016-06-24" @default.
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• W1990268265 date "2014-05-01" @default.
• W1990268265 modified "2023-10-01" @default.
• W1990268265 title "Soluble adenylyl cyclase accounts for high basal cCMP and cUMP concentrations in HEK293 and B103 cells" @default.
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• W1990268265 doi "https://doi.org/10.1016/j.bbrc.2014.04.099" @default.
• W1990268265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24792377" @default.
• W1990268265 hasPublicationYear "2014" @default.
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