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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990300178> ?p ?o ?g. }

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• W1990300178 abstract "Gel-filtration is commonly used to remove contaminants from conventional actin prepared by the method of Spudich and Watt. It has been shown that this procedure removes the majority of a factor that reduces the low-shear viscosity of actin. We have previously reported that this factor is Cap Z, a barbed end capping protein. We now establish that, even after gel-filtration, enough Cap Z can be present in conventionally prepared actin to affect events occurring at the barbed ends of actin filaments. We also demonstrate that the concentration of Cap Z can be reduced to more than a log below the KD for binding of Cap Z to actin by either (1) immunoabsorbtion of conventionally prepared actin with anti-Cap Z antibodies, or (2) an additional cycle of polymerization/depolymerization followed by repeat gel-filtration. © 1995 Wiley-Liss, Inc." @default.
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• W1990300178 date "1995-01-01" @default.
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• W1990300178 title "Quantitation of cap Z in conventional actin preparations and methods for further purification of actin" @default.
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• W1990300178 doi "https://doi.org/10.1002/cm.970300208" @default.
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