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• W1990306069 endingPage "1615" @default.
• W1990306069 startingPage "1604" @default.
• W1990306069 abstract "The performance of columns packed with the new 2.6 μm Kinetex-C18 shell particles was investigated in gradient elution chromatography and compared with those of the 2.7 μm Halo-C18 shell particles and the 1.7 μm BEH-C18 totally porous particles. The peak capacities Pc of these columns were derived from the resolution of the components of a peptide mixture (β-Lactoglobulin digest) and of a mixture of two biomolecules (insulin and lyzozyme).The three columns exhibit the same peak capacities for the peptides at low linear velocity (u0< 0.05 cm/s) and at any gradient steepness (0.8 <G< 10). When the linear velocity is increased 10-fold, the peak capacity of the Kinetex column remains nearly unchanged while those of the Halo-C18 and the BEH-C18 columns decrease by 20%, approximately. This result confirms the very flat HETP curve, the very low C term of the Kinetex column and its ability to successfully operate at high flow rates while experiencing less efficiency loss than other columns. Despite its smaller average mesopore size (96 Å versus 130 Å), the column packed with 2.6 μm shell Kinetex-C18 particles gives an equivalent or even slightly better separation of biomolecules having a size and a mass around 40 Å and 15 kDa, respectively, than the column packed with 1.7 μm BEH-C18 totally porous particles. This result demonstrates the advantages of the shell versus the conventional particle technology when it comes to resolve mixtures of large and slow diffusive biomolecules." @default.
• W1990306069 created "2016-06-24" @default.
• W1990306069 creator A5038183935 @default.
• W1990306069 creator A5089250649 @default.
• W1990306069 date "2010-03-01" @default.
• W1990306069 modified "2023-10-07" @default.
• W1990306069 title "Performance of columns packed with the new shell Kinetex-C18 particles in gradient elution chromatography" @default.
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• W1990306069 doi "https://doi.org/10.1016/j.chroma.2010.01.008" @default.
• W1990306069 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20122688" @default.
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