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• W1990308825 endingPage "221" @default.
• W1990308825 startingPage "211" @default.
• W1990308825 abstract "A thymic epithelial cell line Mm2T was cultured in a medium containing a high concentration (100μg/ml) of methylated vitamin B12 (CH3-B12). After 19 days, cells were found to have a flat phenotype, to have lost the floating cells which were observed in the control cells at the con fluent stage, and to have acquired a resistance to trypsin. However, treatment of the CH3-B12-treated cells with EDTA resulted in a dissociation of cell-to-cell contact and reaggregation was achieved by addition of Ca2+, indicating the involvement of Ca2+ ion in cell-to-cell contact. Electron microscopic analyses revealed that the CH3-B12-treated cells were nearly square in their vertical section, which was in contrast to the dome-shaped feature of the control cells, and their cell-to-cell contact area was significantly widespread, as compared to those of the control, indicating that Mm2T cells acquires an adhesive property by treatment with CH3-B12. Biochemical analyses of both cells indicated that the concentration of glucosylceramide in the CH3-B12-treated cells was higher than that of the control. Free glucose characteristically inhibited the reattachment of cells dissociated with EDTA, suggesting the involvement of glucose in the cell-to-cell adhesion of CH3-B12-treated cells. In addition, WGA-binding glycoconjugates were intensely observed in the boundary region of CH3-B12-treated cells by immunohistochemical staining, but not in that of the control cells. It is suggested that CH3-B12 may affect the morphological alteration of Mm2T by enhancing cell adhesion through elevated expression of the C-type lectin." @default.
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• W1990308825 date "1995-01-01" @default.
• W1990308825 modified "2023-09-30" @default.
• W1990308825 title "Enhancement of Adhesive Property of Epithelial Cell Line Mm2T by Culture in the Presence of Methylated vitamin B12." @default.
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• W1990308825 doi "https://doi.org/10.1247/csf.20.211" @default.
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