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• W1990341264 abstract "Article1 March 2002free access The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surface Norman R. Watts Norman R. Watts Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author James F. Conway James F. Conway Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Institut de Biologie Structurale J.-P.Ebel, 41 rue Jules Horowitz, F-38027 Grenoble, France Search for more papers by this author Naiqian Cheng Naiqian Cheng Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Stephen J. Stahl Stephen J. Stahl Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author David M. Belnap David M. Belnap Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Alasdair C. Steven Corresponding Author Alasdair C. Steven Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Paul T. Wingfield Paul T. Wingfield Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Norman R. Watts Norman R. Watts Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author James F. Conway James F. Conway Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Institut de Biologie Structurale J.-P.Ebel, 41 rue Jules Horowitz, F-38027 Grenoble, France Search for more papers by this author Naiqian Cheng Naiqian Cheng Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Stephen J. Stahl Stephen J. Stahl Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author David M. Belnap David M. Belnap Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Alasdair C. Steven Corresponding Author Alasdair C. Steven Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Paul T. Wingfield Paul T. Wingfield Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA Search for more papers by this author Author Information Norman R. Watts1, James F. Conway2,3, Naiqian Cheng2, Stephen J. Stahl1, David M. Belnap2, Alasdair C. Steven 2 and Paul T. Wingfield1 1Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA 2Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South drive MSC 8025, National Institutes of Health, Bethesda, MD, 20892-8025 USA 3Institut de Biologie Structurale J.-P.Ebel, 41 rue Jules Horowitz, F-38027 Grenoble, France *Corresponding author. E-mail: [email protected] The EMBO Journal (2002)21:876-884https://doi.org/10.1093/emboj/21.5.876 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Many capsid proteins have peptides that influence their assembly. In hepatitis B virus capsid protein, the peptide STLPETTVV, linking the shell-forming ‘core’ domain and the nucleic acid-binding ‘protamine’ domain, has such a role. We have studied its morphogenic properties by permuting its sequence, substituting it with an extraneous peptide, deleting it to directly fuse the core and protamine domains and assembling core domain dimers with added linker peptides. The peptide was found to be necessary for the assembly of protamine domain-containing capsids, although its size-determining effect tolerates some modifications. Although largely invisible in a capsid crystal structure, we could visualize linker peptides by cryo-EM difference imaging: they emerge on the inner surface and extend from the capsid protein dimer interface towards the adjacent symmetry axis. A closely sequence-similar peptide in cellobiose dehydrogenase, which has an extended conformation, offers a plausible prototype. We propose that linker peptides are attached to the capsid inner surface as hinged struts, forming a mobile array, an arrangement with implications for morphogenesis and the management of encapsidated nucleic acid. Introduction Hepatitis B virus (HBV) is responsible for liver disease on a massive scale (Blumberg, 1997). Its virion consists of a nucleocapsid enclosed within an envelope containing the viral glycoprotein, S-antigen. The capsid constitutes the ‘core’ antigen, and an unassembled variant of the same protein forms another major clinical antigen: e-antigen (Milich et al., 1995; Schodel et al., 1996). Nucleocapsids are fenestrated icosahedral particles of two sizes, with triangulation (T) numbers of 3 (180 subunits) and 4 (240 subunits), respectively (Crowther et al., 1994; Wingfield et al., 1995). The capsid protein has a ‘core’ domain, and a ‘protamine’ domain (Petit and Pilot, 1985; Nassal, 1992). Polymerized core domains form the capsid shell, while the protamine domain, residues 150–183 and ∼50% arginine, binds nucleic acid, initially the RNA pregenome, and latterly, the DNA genome. Core domain forms dimers (Wingfield et al., 1995) that assemble efficiently into capsids, both in vivo and in vitro (Cohen and Richmond, 1982; Wingfield et al., 1995). The dimer has the overall shape of a capital T whose stem extends outwards as a spike and the tips of whose arms cluster together in five- or six-membered rings around the symmetry axes of the surface lattice (Crowther et al., 1994). The spike is a four-helix bundle of paired helical hairpins (Zlotnick et al., 1996; Conway et al., 1997), and the core domain fold has been determined (Böttcher et al., 1997; Wynne et al., 1999). Cp140, the shortest core domain shown to form capsids, terminates at residue 140 (Salfeld et al., 1989; Birnbaum and Nassal, 1990), and the first arginine in the protamine domain is at position 150. The intervening nonapeptide is a linker connecting the two domains. The last residue that is sufficiently well ordered to be consistently seen in the crystal structure of the Cp149 capsid (core domain plus linker) is Thr142 (Wynne et al., 1999). However, there are indications that the rest of the linker peptide is not entirely disordered. First, its length and sequence are closely conserved (Bringas, 1997). Secondly, it is a major morphogenic determinant: progressive truncation from the C-terminus reduces the fraction of T = 4 capsids from ∼95% for Cp149 to ∼20% for Cp140 (Zlotnick et al., 1996). The location of the linker peptide has been controversial. In RNA-free capsids with large moieties appended to the core domain, these moieties are accessible to antibodies (Stahl and Murray, 1989; Yoshikawa et al., 1993), implying that at least some of them are on the outside, and the protamine domain is susceptible to proteolysis by enzymes too large to pass through the holes in the surface lattice (Gallina et al., 1989). On the other hand, encapsidated RNA has been depicted as an inner shell (Crowther et al., 1994; Zlotnick et al., 1997), and it is plausible that protamine domains are bound to it. This consideration places the linker peptides inside the capsid. Moreover, cryo-electron microscopy (cryo-EM) observations of capsids labeled with heavy metal clusters attached to a cysteine appended at position 150 (Zlotnick et al., 1997; Cheng et al., 1999) indicate that this peptide is on the inside. The goals of the present study were to define the location of the linker peptide and to further explore its properties. To determine the margin of the core domain and conversely, the N-terminal limit of the linker peptide, we examined the assembly properties of Cp138 and Cp139. To investigate the morphogenic effect of the linker, we substituted it with peptides of the same length—both by scrambling the native sequence and by replacing it with an entirely different sequence. In addition, we examined the effect of added linker peptide on in vitro assembly of Cp142, which lacks most of the linker, and the assembly of a linker-deleted construct in which the protamine domain is fused directly to the core domain. To localize the linker in core domain capsids, we used cryo-EM-based difference mapping between Cp149 and Cp140 particles, an approach used previously to visualize a peptide of similar length at the N-terminus of the core domain (Conway et al., 1998b). Finally, we searched the protein database for peptides with similar sequences in proteins whose structures have been solved, and found such a peptide in cellobiose dehydrogenase of Phanerochaete chrysosporium (Hallberg et al., 2000). This peptide, which is surface exposed and has an extended conformation, was docked into our difference densities. These observations are discussed in terms of the multiple functions attributed to the linker peptide: in morphogenesis; in serving as a spacer; and in organizing the interactions between the protein shell and nucleic acid during replication and transcription. Results Expression of Cp138 and Cp139 When expressed in Escherichia coli, Cp138 and Cp139 produced insoluble aggregates, unlike Cp140–Cp149, which form capsids and/or dimers. These aggregates resisted extraction with strong chaotropes such as 8 M guanidine hydrochloride but were readily solubilized by the anionic detergent SDS or sarkosyl (N-laurylsarcosine). Cp138 was purified by gel filtration in the presence of anionic detergent followed by reversed phase chromatography. The purified protein was dialyzed against 50 mM Tris–HCl pH 7.5, with 250 mM sodium chloride, giving an optically clear solution. The far-UV CD spectrum indicated ∼40% α-helix, not much less than the 55–60% of Cp144 capsids and dimers (Wingfield et al., 1995). Sedimentation velocity analysis indicated some size heterogeneity, with 80% of the material having a median S value of 6.8, corresponding to a mass of ∼100 kDa, and 10–15% sedimenting at 11.2S. In comparison, Cp149 dimers sediment at 2.5S. These results suggest that Cp138 is capable of folding into a conformation resembling that of native core domain and of oligomerizing, but not of ordered polymerization into capsids. Assembly and visualization of Cp140 capsids Expression of Cp140 in E.coli followed the usual pattern, i.e. most of the protein assembled into particles that elute separately from the bulk of soluble bacterial protein during gel filtration (Zlotnick et al., 1996). On shortening the linker, we have observed a progressive increase in the amount of unassembled capsid protein from essentially zero for Cp149 to 10–15% for Cp140. It was our experience that Cp140 is more liable to aggregation than other truncated constructs, e.g. Cp142–Cp149; nevertheless, by not over-concentrating and appropriately adjusting the buffer conditions, especially controlling the pH and including 50 mM Mg2+, we were able to assemble and purify capsids. A cryo-micrograph of Cp140 capsids is shown in Figure 1A, and compared with a field of Cp149 capsids (Figure 1B): as expected for Cp140, the smaller T = 3 capsids predominate. Cp140 produces a higher incidence of malformed or incomplete capsids than do other Cp constructs; nevertheless, enough intact capsids of both sizes were imaged to provide adequate data sets for reconstruction. The final density map of the T = 3 capsid (Figure 1C) combined 914 particles. In parallel, we reconstructed the T = 4 capsid (Figure 1D), although fewer particles were available (292 were eventually combined). These density maps were calculated to 10 Å resolution and in both, the four α-helices per spike (center-to-center spacing, ∼10 Å) were resolved in transverse sections (data not shown). Figure 1.(A and B) Cryo-electron micrographs of Cp140 and Cp149 capsids. Bar = 250 Å. In each case, examples of a T = 3 and a T = 4 capsid are shown at higher magnification (×3) in the inserts. (C and D) Surface renderings of the outside of Cp140 capsids: (C) T = 3; (D) T = 4. Bar = 50 Å. Download figure Download PowerPoint Visualization of linker peptides by difference imaging To identify density specifically associated with the linker peptide, we performed difference imaging between Cp149 and Cp140 capsids. Central sections through the two T = 3 maps are compared in Figure 2A and B, and for the T = 4 maps in Figure 2D and E. Because most Cp149 capsids are T = 4, in this case it was the T = 4 density map that had less noise. Each pair of sections matches up well, and corresponding sections through both difference maps consistently show positive density at specific sites on the inner surface (arrows in Figure 2C and F). These additional densities are not so conspicuous in the Cp149 maps because they lie in the interference fringe of ‘negative density’ that persists because of limitations in our procedure for correcting phase contrast effects and the limited resolution (i.e. Fourier truncation effect). In the difference maps, these fringes largely cancel out, making extra densities present on Cp149 more evident; the densities of interest are stronger than the residual noise peaks, and they are consistently observed at the same sites on independently calculated subunits. Figure 2.Central sections through T = 3 capsids viewed along a 2-fold axis. The red arrows mark one of the locations at which extra density is present in the Cp149 capsid (A) but not the Cp140 capsid (B). This density is also present at other quasi-equivalent, independently calculated, sites on the icosahedral surface lattice. These densities are more conspicuous in the corresponding section through the difference map (C). Bar = 100 Å. (D–F) show the corresponding sections for T = 4 capsids. Download figure Download PowerPoint The extra densities on the inner surface of Cp149 capsids are discernible (cf. Figures 3A and B for T = 3 and 4A and B for T = 4), but are seen more readily when the difference densities are juxtaposed on the Cp140 capsids and rendered in a different color (pink). The 5-fold clusters at the vertices of the two capsids are virtually identical in shape and orientation (cf. ringed densities in Figures 3C and 4C), affording further evidence of their authenticity. Similar features are also present around the local 6-fold axes. Here, there is some suggestion of pair-wise interactions between neighboring linker peptides, but this aspect is near the noise level. Figure 3.(A and B) Interior surfaces of the T = 3 capsids of Cp140 and Cp149. Additional densities present on Cp149 are marked with a red arrow and ring. (C) Stereo rendering of the Cp140 capsid with the difference densities juxtaposed and contoured at the same level (pink). Difference density at the 5-fold is marked with a blue ring. Bar = 100 Å. Download figure Download PowerPoint Figure 4.(A and B) Interior surfaces of the T = 4 capsids of Cp140 and Cp149. Additional densities present on Cp149 are marked with a red arrow and a ring. (C) Stereo rendering of the Cp140 capsid with the difference densities juxtaposed and contoured at the same level (pink). Difference density at the 5-fold is marked with a blue ring. Bar = 100 Å. Download figure Download PowerPoint Effect on assembly of permuting the linker peptide One idea for how the linker exerts its morphogenic influence on the assembly of core domain capsids has been molecular crowding of these peptides as dimers congregate around symmetry axes of nascent capsids (Zlotnick et al., 1996): the larger this peptide, the higher the preponderance of 6-fold rings over 5-fold rings and consequently, the higher the proportion of T = 4 capsids. To test this hypothesis, we permuted the last seven amino acids of the linker to ETVPVLT, anticipating that a peptide of the same size and amino acid composition might behave in much the same way, according to this ‘space-filling’ hypothesis. The first two linker residues, having a defined conformation in the crystal structure, were left unchanged. A negatively stained electron micrograph of this protein, Cp142ETVPVLT, as extracted from E.coli, is shown in Figure 5A. There are very few capsids: the protein appears to be aggregating laterally, but not in a manner orderly enough to produce closed shells. Figure 5.Effects on assembly of substituting the last seven residues of linker peptide. (A) Cp142ETVPVLT, permuting the native sequence, substitutes a heptapeptide of the same size, net charge and polarity. Under conditions conducive to capsid assembly, most of the material forms aggregates, shown here by negative staining. In places, its appearance suggests a condensate of partially formed capsids. Very few complete capsids are present (arrowheads). (B) Substitution with the N-terminal heptapeptide. This construct, Cp142MDIDPYK, assembled normally, producing almost exclusively T = 4 particles as illustrated here by cryo-EM. Bar = 1000 Å. Download figure Download PowerPoint Effect on assembly of deleting the linker peptide Cp140 core domains lack the linker but are assembly competent (see above). To test whether this property is retained in the presence of the protamine domain, we created a construct, Cp183Δ143−149, in which the Cp140 core domain is fused directly to the protamine domain. If this protein were to assemble into capsids when expressed in E.coli, we would expect it to encapsidate bacterial RNA, and if the morphogenic proclivity of Cp140 is retained, these nucleocapsids should be mainly T = 3. However, Cp183Δ143−149 did not produce capsids. It may be that without the linker to serve as a spacer, the protamine domains, possibly with bound RNA, intrude into the inter-dimer interaction surfaces, subverting assembly. Effect on assembly of substituting the linker peptide with the N-terminal peptide We also performed an assembly experiment in which the linker was substituted by an entirely different peptide of the same size. Confronted with 207 options for residues 143–149, we chose to use the N-terminus of the same protein—MDIDPYK—on the grounds that this peptide does not sabotage assembly when situated at the other end of the polypeptide chain. The resulting construct, Cp142MDIDPYK, assembled adequately, as confirmed by negative staining (not shown). The T = 3:T = 4 ratio of these particles was determined by cryo-EM (e.g. Figure 5B). Essentially all intact capsids (i.e. >99%) were T = 4. When this material was dissociated to dimers with 2 M urea and reassembled in vitro, there were ∼90% T = 4 capsids and ∼10% T = 3 capsids, like Cp149. The latter observation also indicates that, as noted earlier (Zlotnick et al., 1996), in vitro assembly of capsid protein constructs can be affected by their previous history. Effect on Cp140 assembly of added linker peptide In principle, the morphogenic effect of linker peptide may involve both linker–linker interactions and interactions between linker and core domain. To test for the latter, we assembled purified Cp140 dimers (which normally produce ∼20% T = 4 capsids)in vitro in the presence of an excess of linker peptide. If linker–core interactions predominate, the peptide would be expected to increase the proportion of T = 4 peptides (>90% are produced by Cp149). However, no such effect was observed. The fraction of T = 4 capsids obtained with this experiment was unchanged according to both negative staining (21%; N = 1375) and cryo-EM (19%; N = 329). This experiment does not rule out linker–core domain interactions, but it implies that the linker has to be appropriately tethered to the core domain for such effects to be expressed. A similar peptide in the crystal structure of cellobiose dehydrogenase To ascertain whether any similar peptides are present in known structures, we scanned the SwissProt database with FASTA (Pearson and Lipman, 1988) and found such a peptide in residues 103–110 of cellobiose dehydrogenase (Hallberg et al., 2000). When its sequence, TTLPETTI, is compared with the first eight residues of the HBV linker, the central six are identical and the two flanking amino acids are chemically similar, i.e. T for S and I for V. In cellobiose dehydrogenase, this peptide is surface-exposed and has an extended conformation with no regular (i.e. α- or β-) secondary structure (Figure 6A). Figure 6.Modeling a peptide structure into linker-associated densities visualized by cryo-EM difference imaging. Residues 103–110 of cellobiose dehydrogenase (Hallberg et al., 2000) are almost completely identical to the linker sequences (A). In (B), this peptide is shown (white α-carbon trace) docked into the difference density (pink mesh) from the T = 4 capsids. Quasi-equivalent core domain subunits are shown in green, yellow, red and blue. Five-fold (at left) and 2-fold (at right) symmetry axes are marked. In (C), the view is from the inside of the capsid. We propose that a semi-flexible hinge allows the linker to pivot, so that it serves as a spacer that prevents the protamine domains from intruding too closely into inter-core domain contacts, and also confers mobility on the protamine domains' interactions with nucleic acid. Download figure Download PowerPoint Conformational analysis of linker peptides We measured the CD spectra of two synthetic peptides corresponding to residues 139–149 and 141–149, respectively. Both spectra were indicative of random coil without detectable content of regular secondary structure (data not shown), emulating the cellobiose dehydrogenase peptide in this respect. We also tried to assess linker conformation in capsids by differential CD analysis between Cp149 and Cp142, but the results were inconclusive because they differ so little in size (5%). Molecular modeling of difference densities This peptide was grafted on to quasi-atomic capsid models obtained by fitting the atomic models of the dimers (Wynne et al., 1999) into our cryo-EM density maps of the T = 4 and T = 3 capsids (Figures 6B and C and 7A). The first three residues of the cellobiose dehydrogenase peptide were overlaid on the last three residues seen in the capsid protein (residues 141–143) and fitted well, confirming that they have similar conformations. This operation fixed the position of the rest of the peptide, which matched the observed difference densities quite well at each of the seven sites, i.e. it was at least partially inside or grazing them. The docking was then refined to position the peptides more centrally in the difference densities. Given their limited resolution, there was some ambiguity about how this should be done. The fits shown in Figure 6B and C were obtained by pivoting about residue 143, the putative hinge site (see Discussion), by angles that varied from ∼20° to ∼50°, depending on the quasi-equivalent site in question. Figure 7.(A) Quasi-atomic model of the T = 3 HBV Cp142 capsid. The model was created by docking dimers from the crystal structure of the T = 4 capsid into a cryo-EM map of the T = 3 capsid. (B and D) Central sections through quasi-atomic models of the T = 3 (B) and T = 4 (D) capsids. Cp142 capsids, restricted to 10 Å resolution, closely resemble the corresponding sections through the cryo-EM density maps of Cp149 and Cp140 (cf. Figures 2A, B, D and E). Corresponding sections through quasi-atomic models of the icosahedrally coordinated linker peptide (represented by the cellobiose dehydrogenase peptide, appropriately docked) are shown in (C) and (E) (cf. Figure 2C and F). Bar = 100 Å. Download figure Download PowerPoint Discussion Some capsid proteins have, in addition to a basic building block or core domain, peptides that regulate assembly and can affect the size of capsid produced. The N-terminus of papillomavirus L1 is one example (Chen et al., 2000). This mechanism is not exclusive to virus capsids and is also operative, for instance, in the assembly of bacterial flagellae (Namba, 2001). Other capsid proteins have positively charged extensions that bind nucleic acid (e.g. Vriend et al., 1986). HBV, with its C-terminal protamine domain connected by the linker peptide to the core domain, has both features. In this study, we have examined structural, conformational and morphogenic properties of its linker peptide. Minimum-sized core domain for correct folding It has previously been reported that Cp138 is not assembly competent (Birnbaum and Nassal, 1990; Beames and Lanford, 1993; Metzger and Bringas, 1998), and we have found that Cp139 shares this property. Moreover, a monoclonal antibody that bound to Cp140 failed to react with Cp138 (Salfeld et al., 1989), suggesting a different conformation. Our experiments were intended to determine whether this breakdown in assembly reflects a failure to produce functional building blocks or a failure of building blocks to form capsids. We found that Cp138 could assemble into soluble multimers, rich in α-helix, but not into capsids. This observation suggests that with Cp138, the dimer interface, the four-helix bundle, may be formed correctly, but incompleteness of its arms, which engage in the inter-dimer contacts, precludes assembly into capsids. Wynne et al. (1999) noted a snug interaction involving Leu140 in their crystal structure of Cp149 T = 4 capsids, and inferred that this was a key interaction to stabilizing the protein fold. Our data with the Cp138 and 139 are consistent with this proposal, which is further supported by the observation that the mutant Cp140.L140A is unstable and also produces insoluble aggregates when expressed in E.coli (P.T.Wingfield and S.J.Stahl, unpublished data). Expression characteristics and capsid stability The various HBV capsid protein constructs that we have expressed exhibit three physical states: capsids, dimers and aggregates. Whereas Cp183 (full-length protein) assembles efficiently into capsids, most shorter constructs leave some unassembled dimers, ranging up to 10–15% for Cp140. We use gel filtration to resolve capsids from dimers and soluble bacterial proteins. In general, the amount of unassembled protein correlates with instability of the parent capsid (P.T.Wingfield and N.R.Watts, unpublished results). Thus, Cp140 capsids are somewhat fragile and aggregation prone compared with the very stable Cp149 capsids. Instead of capsids, Cp138, Cp142ETVPVLT and Cp183Δ143−149 produce insoluble aggregates, typical of the inclusion bodies formed when many proteins are overexpressed in E.coli. Location of the linker peptide Prior evidence is strong that the linker is on the inside when attached to the protamine domain–RNA complex or metal clusters (see Introduction), but in the absence of such constraints, the data are less conclusive. Uncon strained linkers may, in principle, fluctuate between internal and external positions as in the ‘breathing’ motions attributed to capsid protein peptides of rhinovirus (Smith et al., 1993) and poliovirus (Li et al., 1994). In this context, our difference mapping visualizes extended, linker-associated, densities at specific sites on the inner surface. These linkers are in essentially the same position as when bound to undecagold (Zlotnick et al., 1997) or tetrairidium (Cheng et al., 1999), indicating that their placement reflects properties of the linker per se and not of the ligands. While these data do not rule out the possibility that linkers fluctuate between the inner and outer surfaces, they establish that the linkers spend most of the time in more-or-less defined positions on the inner surface. Visualizing pe" @default.
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• W1990341264 title "The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surface" @default.
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