FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990355896> ?p ?o ?g. }

• W1990355896 endingPage "3827" @default.
• W1990355896 startingPage "3826" @default.
• W1990355896 abstract "The increasing prevalence of antimicrobial resistance requires a continuous effort to find new antimicrobials. The availability of genome sequences for a variety of microbes has facilitated the development of whole-genome, single-gene knockout libraries for systematic gene function analysis. These libraries, such as the Keio library of Escherichia coli (1) and the University of Washington two-allele library of Pseudomonas aeruginosa (3), can be used in high-throughput phenotypic array studies (7); thus, they seem well suited for identifying new antimicrobial targets. However, we recently noticed with the P. aeruginosa two-allele mutant library that intrinsic, unidentified factors may affect susceptibility for some antimicrobials with many randomly picked mutants, thereby obscuring effects attributed to specific candidate mutants.In E. coli, a phoU knockout mutation confers hypersensitivity to a variety of antimicrobials and other stresses (5). To determine whether inactivation of a phoU homologue in P. aeruginosa confers a similar phenotype, we obtained a phoU mutant and the parental wild-type strain (PAO1, lacking a transposon insertion) from the University of Washington two-allele library. When quinolone susceptibility was tested, a 4- to 16-fold decrease in MIC was observed for both ciprofloxacin and oxolinic acid (Table ​(Table1),1), as expected (5). However, when 10 unrelated mutants were tested, each of those mutants also exhibited hypersusceptibility to quinolones (Table ​(Table1).1). Moreover, PCR analysis failed to confirm transposition being inside the phoU allele. Thus, increased susceptibility of the phoU mutant could not be specifically attributed to the phoU mutation.TABLE 1.Antimicrobial susceptibility of mutants obtained from the P. aeruginosa two-allele libraryColony color with most of these mutants was darker (bluish green) than with the wild-type strain, indicating production of more phenazine pigments in the mutants. Since phenazines, such as pyocyanin, may be involved in oxidative stress and thereby affect antimicrobial susceptibility (2), we tested two additional mutants (the phzS and phzM mutants) that do not produce pyocyanin (6). Reduced MICs, similar to those seen with the other mutants, were observed. Thus, variation in pyocyanin production was not responsible for reduced quinolone susceptibility. Since the mutants tested had transposon insertions at a variety of chromosomal locations, some of which were far outside open reading frames, transposon-mediated polarity is also an unlikely explanation for reduced MIC. When other antimicrobial classes were tested, hypersusceptibility was observed with chloramphenicol (4- to 64-fold) but not with tobramycin, meropenem, or ceftazidime (Table ​(Table1).1). Thus, the mutants tested were universally hypersusceptible to some, but not all, antimicrobials for reasons that are unknown. This lack of specificity may not be unique to the University of Washington library, because several lux-insertional mutants obtained from a different library (4) also conferred quinolone hypersusceptibility (data not shown).The results described above suggest that the transposition/insertion of a DNA fragment into the P. aeruginosa chromosome may itself have a general effect on susceptibility for some, but not all, antimicrobials. Had we simply compared the phoU mutant with the parental wild-type strain, which is standard practice with many antimicrobial studies, we would have been misled. Thus, interpretation of antimicrobial experiments involving mutants from the P. aeruginosa insertional libraries requires caution." @default.
• W1990355896 created "2016-06-24" @default.
• W1990355896 creator A5030578205 @default.
• W1990355896 creator A5067771631 @default.
• W1990355896 creator A5073279939 @default.
• W1990355896 date "2008-10-01" @default.
• W1990355896 modified "2023-10-16" @default.
• W1990355896 title "Antimicrobial Studies with the Pseudomonas aeruginosa Two-Allele Library Require Caution" @default.
• W1990355896 cites W2068651456 @default.
• W1990355896 cites W2093262908 @default.
• W1990355896 cites W2111967267 @default.
• W1990355896 cites W2126918333 @default.
• W1990355896 cites W2152193238 @default.
• W1990355896 cites W2154063145 @default.
• W1990355896 cites W2168207658 @default.
• W1990355896 doi "https://doi.org/10.1128/aac.00419-08" @default.
• W1990355896 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2565873" @default.
• W1990355896 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18694947" @default.
• W1990355896 hasPublicationYear "2008" @default.
• W1990355896 type Work @default.
• W1990355896 sameAs 1990355896 @default.
• W1990355896 citedByCount "4" @default.
• W1990355896 countsByYear W19903558962013 @default.
• W1990355896 countsByYear W19903558962017 @default.
• W1990355896 countsByYear W19903558962018 @default.
• W1990355896 crossrefType "journal-article" @default.
• W1990355896 hasAuthorship W1990355896A5030578205 @default.
• W1990355896 hasAuthorship W1990355896A5067771631 @default.
• W1990355896 hasAuthorship W1990355896A5073279939 @default.
• W1990355896 hasBestOaLocation W19903558961 @default.
• W1990355896 hasConcept C104317684 @default.
• W1990355896 hasConcept C143065580 @default.
• W1990355896 hasConcept C180754005 @default.
• W1990355896 hasConcept C2777637488 @default.
• W1990355896 hasConcept C4918238 @default.
• W1990355896 hasConcept C4937899 @default.
• W1990355896 hasConcept C523546767 @default.
• W1990355896 hasConcept C54355233 @default.
• W1990355896 hasConcept C547475151 @default.
• W1990355896 hasConcept C86803240 @default.
• W1990355896 hasConcept C89423630 @default.
• W1990355896 hasConceptScore W1990355896C104317684 @default.
• W1990355896 hasConceptScore W1990355896C143065580 @default.
• W1990355896 hasConceptScore W1990355896C180754005 @default.
• W1990355896 hasConceptScore W1990355896C2777637488 @default.
• W1990355896 hasConceptScore W1990355896C4918238 @default.
• W1990355896 hasConceptScore W1990355896C4937899 @default.
• W1990355896 hasConceptScore W1990355896C523546767 @default.
• W1990355896 hasConceptScore W1990355896C54355233 @default.
• W1990355896 hasConceptScore W1990355896C547475151 @default.
• W1990355896 hasConceptScore W1990355896C86803240 @default.
• W1990355896 hasConceptScore W1990355896C89423630 @default.
• W1990355896 hasIssue "10" @default.
• W1990355896 hasLocation W19903558961 @default.
• W1990355896 hasLocation W19903558962 @default.
• W1990355896 hasLocation W19903558963 @default.
• W1990355896 hasLocation W19903558964 @default.
• W1990355896 hasOpenAccess W1990355896 @default.
• W1990355896 hasPrimaryLocation W19903558961 @default.
• W1990355896 hasRelatedWork W1434634424 @default.
• W1990355896 hasRelatedWork W1481972220 @default.
• W1990355896 hasRelatedWork W1863186938 @default.
• W1990355896 hasRelatedWork W1979654049 @default.
• W1990355896 hasRelatedWork W1996512949 @default.
• W1990355896 hasRelatedWork W2015813873 @default.
• W1990355896 hasRelatedWork W2067849157 @default.
• W1990355896 hasRelatedWork W2069107501 @default.
• W1990355896 hasRelatedWork W2086350630 @default.
• W1990355896 hasRelatedWork W2108429043 @default.
• W1990355896 hasRelatedWork W2134828411 @default.
• W1990355896 hasRelatedWork W2138956870 @default.
• W1990355896 hasRelatedWork W2147530497 @default.
• W1990355896 hasRelatedWork W2148753657 @default.
• W1990355896 hasRelatedWork W2164299830 @default.
• W1990355896 hasRelatedWork W2749660753 @default.
• W1990355896 hasRelatedWork W2922306967 @default.
• W1990355896 hasRelatedWork W2941278472 @default.
• W1990355896 hasRelatedWork W2979705761 @default.
• W1990355896 hasRelatedWork W2994542102 @default.
• W1990355896 hasVolume "52" @default.
• W1990355896 isParatext "false" @default.
• W1990355896 isRetracted "false" @default.
• W1990355896 magId "1990355896" @default.
• W1990355896 workType "article" @default.